Cloned (Comment) | Organism |
---|---|
chcA in pUC18 and pET3C | Streptomyces collinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA | only impure solution available, KM and vmax in same order of magnitude as with 1-cyclohexenylcarbonyl-CoA | Streptomyces collinus | |
0.025 | - |
1-cyclohexenylcarbonyl-CoA | vmax 0.0075 mmol/mg/min | Streptomyces collinus | |
0.03 | - |
5-hydroxycyclohex-1-enecarbonyl-CoA | vmax 0.0053 mmol/mg/min | Streptomyces collinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29700 | - |
ChcA, gene sequence | Streptomyces collinus |
37000 | - |
ChcA, SDS-PAGE, +/-2000 | Streptomyces collinus |
75000 | - |
ChcA, gel filtration on superose 12, +/-10000 | Streptomyces collinus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-cyclohexenylcarbonyl-CoA + NADPH | Streptomyces collinus | reaction in ansatrienin biosynthesis out of shikimic acid | 1-cyclohexylcarbonyl-CoA + NADP+ | - |
? | |
5-hydroxycyclohex-1-enecarbonyl-CoA + NADPH | Streptomyces collinus | reaction in ansatrienin biosynthesis out of shikimic acid | 5-hydroxycyclohexylcarbonyl-CoA + NADP+ | - |
? | |
trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA + NADPH | Streptomyces collinus | reaction in ansatrienin biosynthesis out of shikimic acid | trans-4,5-dihydroxycyclohexa-2-enecarbonyl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces collinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography of protein overexpressed in Escherichia coli by using Sephadex G-100, DEAE, Mono-Q HR 5/5 | Streptomyces collinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.882 | - |
crude Escherichia coli cell extract after overexpression | Streptomyces collinus |
3.667 | - |
purified, overexpressed enzyme | Streptomyces collinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-cyclohexenylcarbonyl-CoA + NADPH | reaction in ansatrienin biosynthesis out of shikimic acid | Streptomyces collinus | 1-cyclohexylcarbonyl-CoA + NADP+ | - |
? | |
5-hydroxycyclohex-1-enecarbonyl-CoA + NADPH | reaction in ansatrienin biosynthesis out of shikimic acid | Streptomyces collinus | 5-hydroxycyclohexylcarbonyl-CoA + NADP+ | - |
? | |
trans-3,4-dihydroxycyclohexa-1,5-dienecarbonyl-CoA + NADPH | reaction in ansatrienin biosynthesis out of shikimic acid | Streptomyces collinus | trans-4,5-dihydroxycyclohexa-2-enecarbonyl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, homodimer, comparison molecular weight SDS-PAGE versus gel filtration | Streptomyces collinus |
Synonyms | Comment | Organism |
---|---|---|
1-cyclohexenylcarbonyl coenzyme A reductase | - |
Streptomyces collinus |