Literature summary for 1.23.5.1 extracted from

Saga, G.; Giorgetti, A.; Fufezan, C.; Giacometti, G.M.; Bassi, R.; Morosinotto, T.
Mutation analysis of violaxanthin de-epoxidase identifies substrate-binding sites and residues involved in catalysis (2010), J. Biol. Chem., 285, 23763-23770.

Search for more literature for 1.23.5.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Origami B cells	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
D114A	inactive	Arabidopsis thaliana
D114N	inactive	Arabidopsis thaliana
D177A	inactive	Arabidopsis thaliana
D177N	inactive	Arabidopsis thaliana
D178A	the mutant shows 56% activity compared to the wild type enzyme	Arabidopsis thaliana
F123A	the mutant shows 34% activity compared to the wild type enzyme	Arabidopsis thaliana
F155A	the mutant shows 5% activity compared to the wild type enzyme	Arabidopsis thaliana
H121A	the mutant shows 5% activity compared to the wild type enzyme	Arabidopsis thaliana
N167A	the mutant shows 121% activity compared to the wild type enzyme	Arabidopsis thaliana
Q119A	the mutant shows 32% activity compared to the wild type enzyme	Arabidopsis thaliana
Q153A	the mutant shows 75% activity compared to the wild type enzyme	Arabidopsis thaliana
Q153E	the mutant shows 60% activity compared to the wild type enzyme	Arabidopsis thaliana
Q153L	the mutant shows 30% activity compared to the wild type enzyme	Arabidopsis thaliana
T245A	the mutant shows 82% activity compared to the wild type enzyme	Arabidopsis thaliana
W179A	the mutant shows less than 2% activity compared to the wild type enzyme	Arabidopsis thaliana
W179N	the mutant shows less than 2% activity compared to the wild type enzyme	Arabidopsis thaliana
Y175F	the mutant shows 62% activity compared to the wild type enzyme	Arabidopsis thaliana
Y198F	inactive	Arabidopsis thaliana
Y214F	the mutant shows less than 2% activity compared to the wild type enzyme	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
pepstatin A	-	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
thylakoid membrane	-	Arabidopsis thaliana	42651	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q39249	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
antheraxanthin + ascorbate	-	Arabidopsis thaliana	zeaxanthin + dehydroascorbate + H2O	-	?
violaxanthin + ascorbate	-	Arabidopsis thaliana	antheraxanthin + dehydroascorbate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
VDE	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)