Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | high concentrations of cofactor increases type I activity | Rattus sp. | |
additional information | thiol cofactor reaction stimulation reduced in type I mutant with exchange of selenocysteine by cysteine | Rattus sp. |
Cloned (Comment) | Organism |
---|---|
expression in JEG-3 cells, transient transfection | Rattus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | exchange selenocysteine to cysteine by site-directed mutagenesis of isoenzyme type I | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-Propyl-2-thiouracil | competes with thiol cofactor; type I mutant with exchange of selenocysteine by cystein is 300fold less sensitive | Rattus sp. | |
additional information | 3,3',5'-triiodothyronine blocks 5'-deiodination of L-thyroxine blocks 5'-deiodination of 3,3',5'-triiodothyronine | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
3,3',5'-triiodothyronine | type I | Rattus sp. | |
0.25 | - |
3,3',5'-triiodothyronine | DTT 30 mM | Rattus sp. | |
2.7 | - |
3,3',5'-triiodothyronine | type I mutant, exchange of selenocysteine by cysteine | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | type I | Rattus sp. | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5'-triiodothyronine + AH2 | Rattus sp. | isoenzyme type I | 3,3'-diiodothyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodothyronine + AH2 | Rattus sp. | isoenzyme type II and type III | 3,3'-diiodothyronine + iodide + A + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | thyronine 5'-deiodinase type I | Rattus sp. | - |
liver | thyronine 5'-deiodinase type I | Rattus sp. | - |
thyroid gland | thyronine 5'-deiodinase type I | Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5'-triiodothyronine + AH2 | isoenzyme type I | Rattus sp. | 3,3'-diiodothyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodothyronine + AH2 | - |
Rattus sp. | 3,3'-diiodothyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodothyronine + AH2 | isoenzyme type II and type III | Rattus sp. | 3,3'-diiodothyronine + iodide + A + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
iodothyronine 5'-deiodinase | type I, selenoprotein, inducible by T3 (higher synthesis) and angiotensin II (positive chronotropic effect in myocard) | Rattus sp. |