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Literature summary for 1.21.99.4 extracted from

  • Auf dem Brinke, D.; Hesch, R.D.; Köhrle, J.
    Re-examination of the subcellular localization of thyroxine 5-deiodination in rat liver (1979), Biochem. J., 180, 273-279.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002 0.0006 L-thyroxine isoform III, 37°C, pH 6.0 Rattus norvegicus
0.001
-
L-thyroxine isoform II, 37°C, pH 6.0 Rattus norvegicus
0.002 0.0035 L-thyroxine isoform I, 37°C, pH 6.8 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome enzyme isoforms I, II Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
enzyme isoforms I, II, and III
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, presence of 3 mM dithiothreitol, stable for at least 6 weeks, after 3 months, loss of55% of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-thyroxine + AH2
-
Rattus norvegicus 3,5,3'-triiodo-L-thyronine + iodide + A + H+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 isoform III Rattus norvegicus
5.8 6.3 isoform II Rattus norvegicus
6.8
-
isoform I Rattus norvegicus