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Literature summary for 1.21.99.3 extracted from
- Presence of growth factors-induced type III iodothyronine 5-deiodinase in cultured rat brown adipocytes (1995), Endocrinology, 136, 4543-4550.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acidic fibroblast growth factor | 50fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
| basic fibroblast growth factor | 45fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
| Epidermal growth factor | 25fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus |  |
| insulin-like growth factor I | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
| platelet-derived growth factor | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
| Vasopressin | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000225 | - |
3,3',5-triiodo-L-thyronine | pH 7.5, 37°C, isozyme type III | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,3',5-triiodo-L-thyronine + AH2 | Rattus norvegicus | no detectable activity in vivo, isozyme type III | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley rats | - |
| Rattus norvegicus | - |
isozyme type III | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | cell culture | Rattus norvegicus | - |
| adipocyte | isozyme type III | Rattus norvegicus | - |
| brown adipose tissue | cell culture | Rattus norvegicus | - |
| brown adipose tissue | isozyme type III | Rattus norvegicus | - |
| cell culture | brown adipocytes | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
effect of growth factors on isozyme type III | Rattus norvegicus |
| 0.000042 | - |
isozyme type III, brown adipocyte cell culture, in presence of 10% newborn calf serum, substrate 3,3',5-triiodo-L-thyronine | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,3',5-triiodo-L-thyronine + AH2 | - |
Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
| 3,3',5-triiodo-L-thyronine + AH2 | no detectable activity in vivo, isozyme type III | Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
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