Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.4.2 extracted from

  • Stadtman, T.C.
    Clostridial glycine reductase: protein C, the acetyl group acceptor, catalyzes the arsenate-dependent decomposition of acetyl phosphate (1989), Proc. Natl. Acad. Sci. USA, 86, 7853-7856.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
arsenate absolutely required for protein C activity Acetoanaerobium sticklandii
thiols such as dithiothreitol required for decomposition of acetyl phosphate by protein C Acetoanaerobium sticklandii

General Stability

General Stability Organism
the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited by alkylation selenoprotein A alkylated at pH 6 with bromoacetate is active as a component of the enzyme complex Acetoanaerobium sticklandii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for decomposition of acetyl phosphate by protein C Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
molecular mass is depending on the salt concentration present Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O Acetoanaerobium sticklandii
-
glycine + phosphate + thioredoxin
-
?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of protein C of enzyme Acetoanaerobium sticklandii

Reaction

Reaction Comment Organism Reaction ID
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin the protein C component catalyses the arsenate-dependent decomposition of acetyl phosphate Acetoanaerobium sticklandii
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin the protein component C serves as the acetyl group acceptor in the overall reaction Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
?
acetyl phosphate + NH3 + thioredoxin disulfide + H2O reduction of glycine action is coupled with formation of ATP from ADP Acetoanaerobium sticklandii glycine + phosphate + thioredoxin
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62 64 protein C inactivated Acetoanaerobium sticklandii