Literature summary for 1.21.4.1 extracted from

Kabisch, U.C.; Grantzdorffer, A.; Schierhorn, A.; Rucknagel, K.P.; Andreesen, J.R.; Pich, A.
Identification of D-proline reductase from Clostridium sticklandii as a selenoenzyme and indications for a catalytically active pyruvoyl group derived from a cysteine residue by cleavage of a proprotein (1999), J. Biol. Chem., 274, 8445-8454.

Search for more literature for 1.21.4.1

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the genes encoding D-proline reductase, 4.8 kb EcoRI fragment containing the genes prdA and prdB isolated and sequenced, prdAcodes for a 68 kDa protein, posttranslationally cleaved to the 45 kDa and 23 kDa subunits, prdB encodes the 26 kDa subunit	Acetoanaerobium sticklandii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Acetoanaerobium sticklandii	5737	-
membrane	-	Acetoanaerobium sticklandii	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for the reaction	Acetoanaerobium sticklandii
Se2+	containing selenium in form of selenocysteine and a carbonyl moiety, most probably a pyruvoyl group	Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry	Acetoanaerobium sticklandii
30000	-	10 * 30000	Acetoanaerobium sticklandii
45000	-	10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry	Acetoanaerobium sticklandii
300000	-	gel filtration	Acetoanaerobium sticklandii
870000	-	gel filtration, native PAGE	Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-proline + NADH	Acetoanaerobium sticklandii	NADH is more effective than dithiols in crude preparations	5-aminopentanoic acid + NAD+	-	?
D-proline + NADH	Acetoanaerobium sticklandii DSM 519	NADH is more effective than dithiols in crude preparations	5-aminopentanoic acid + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Acetoanaerobium sticklandii	-	strain HF, DSM 517 T	-
Acetoanaerobium sticklandii DSM 519	-	strain HF, DSM 517 T	-
Clostridium sporogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
16.1	-	-	Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-proline + dithiol (reduced)	-	Acetoanaerobium sticklandii	5-aminopentanoic acid + oxidized dithiol	-	?
D-proline + dithiol (reduced)	-	Acetoanaerobium sticklandii DSM 519	5-aminopentanoic acid + oxidized dithiol	-	?
D-proline + dithiothreitol	dithiothreitol as artificial electron donor	Acetoanaerobium sticklandii	5-amino pentanoic acid + ?	-	ir
D-proline + dithiothreitol	Stickland reaction	Acetoanaerobium sticklandii	5-amino pentanoic acid + ?	-	ir
D-proline + dithiothreitol	dithiothreitol as artificial electron donor	Acetoanaerobium sticklandii DSM 519	5-amino pentanoic acid + ?	-	ir
D-proline + dithiothreitol	Stickland reaction	Acetoanaerobium sticklandii DSM 519	5-amino pentanoic acid + ?	-	ir
D-proline + NADH	NADH is more effective than dithiols in crude preparations	Acetoanaerobium sticklandii	5-aminopentanoic acid + NAD+	-	?
D-proline + NADH	NADH is more effective than dithiols in crude preparations	Acetoanaerobium sticklandii DSM 519	5-aminopentanoic acid + NAD+	-	?

Subunits

Subunits	Comment	Organism
decamer	10 * 30000	Acetoanaerobium sticklandii
decamer	10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry	Acetoanaerobium sticklandii

Synonyms

Synonyms	Comment	Organism
EC 1.4.1.6	formerly	Acetoanaerobium sticklandii

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Release 2023.1 (January 2023)