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Literature summary for 1.21.4.1 extracted from
- Studies on the enzymic reduction of amino acids. II. Purification and properties of a D-proline reductase and a proline racemase from Clostridium sticklandii (1957), J. Biol. Chem., 228, 983-997.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Acetoanaerobium sticklandii |  |
General Stability
| General Stability | Organism |
|---|---|
| withstands ethanol and acetone fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alkaline phosphomonoesterase | preparation from calf intestine, causes extensive inactivation | Acetoanaerobium sticklandii | |
| arsenite | - |
Acetoanaerobium sticklandii | |
| Co2+ | - |
Acetoanaerobium sticklandii | |
| Delta-Aminovalerate | - |
Acetoanaerobium sticklandii | |
| Fe2+ | - |
Acetoanaerobium sticklandii | |
| human seminal plasma | 50% inactivation by incubation at pH 5.5 for 90 min | Acetoanaerobium sticklandii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dialyzed enzyme stimulated by | Acetoanaerobium sticklandii | |
| Mg2+ | depend on | Acetoanaerobium sticklandii | |
| Mn2+ | dialyzed enzyme stimulated by | Acetoanaerobium sticklandii | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
| Ethanol | withstands fractionation at -20°C, does not lose activity as a result of prolonged dialysis | Acetoanaerobium sticklandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
formerly referred to as "an amino acid-fermenting Clostridium, strain HF" | - |
| Clostridium sporogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.4 | - |
- |
Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allohydroxyproline + dihydrolipoate | barely detectable activity | Acetoanaerobium sticklandii | 5-amino-4-hydroxypentanoic acid + lipoate | - |
ir | |
| D-proline + 1,3-dimercaptopropanol | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
| D-proline + 1,3-dimercaptopropanol | oxidation of 1,3-dimercaptopropanol cannot be replaced by molecular hydrogen, NADH+ or a number of other reducing agents | Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
| D-proline + 6,8-dimercaptooctanoate | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
| D-proline + beta-mercaptoethanol | - |
Acetoanaerobium sticklandii | ? | - |
ir | |
| D-proline + dihydrolipoate | - |
Acetoanaerobium sticklandii | delta-aminovaleric acid + lipoate | - |
ir | |
| D-proline + dihydrolipoate | - |
Clostridium sporogenes | delta-aminovaleric acid + lipoate | - |
ir | |
| D-proline + dithiol (reduced) | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + oxidized dithiol | - |
ir | |
| D-proline + DL-lipoic acid | - |
Acetoanaerobium sticklandii | 5-aminopentanoic acid + ? | - |
ir | |
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