Literature summary for 1.21.3.7 extracted from

Sirikantaramas, S.; Morimoto, S.; Shoyama, Y.; Ishikawa, Y.; Wada, Y.; Shoyama, Y.; Taura, F.
The gene controlling marijuana psychoactivity: molecular cloning and heterologous expression of DELTA1-tetrahydrocannabinolic acid synthase from Cannabis sativa L. (2004), J. Biol. Chem., 279, 39767-39774.

Search for more literature for 1.21.3.7

Cloned(Commentary)

Cloned (Comment)	Organism
using a baculovirus-Sf9 insect cell expression system and expressed in Nicotiana tabacum hairy roots	Cannabis sativa

Protein Variants

Protein Variants	Comment	Organism
H114A	the inactive mutant contains no FAD	Cannabis sativa
H208A	the mutant shows 94% wild type activity	Cannabis sativa
H92A	the mutant shows 87% wild type activity	Cannabis sativa
R108A	the mutant shows 13% wild type activity	Cannabis sativa
R110A	the mutant shows 10% wild type activity	Cannabis sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.446	-	cannabigerolate	mutant enzyme R108A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.54	-	cannabigerolate	wild type enzyme, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.783	-	cannabigerolate	mutant enzyme R110A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58597	-	x * 58597, mature protein, calculate from amino acid sequence	Cannabis sativa
60000	-	x * 60000, recombinant enzyme, SDS-PAGE	Cannabis sativa
75000	-	x * 75000, native enzyme, SDS-PAGE	Cannabis sativa

Organism

Organism	UniProt	Comment	Textmining
Cannabis sativa	Q8GTB6	Mexican strain	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme contains eight possible Asn glycosylation sites	Cannabis sativa

Purification (Commentary)

Purification (Comment)	Organism
hydroxylapatite column chromatography	Cannabis sativa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Cannabis sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cannabigerolate + O2	-	Cannabis sativa	DELTA9-tetrahydrocannabinolate + H2O2	-	ir

Subunits

Subunits	Comment	Organism
?	x * 60000, recombinant enzyme, SDS-PAGE	Cannabis sativa
?	x * 75000, native enzyme, SDS-PAGE	Cannabis sativa
?	x * 58597, mature protein, calculate from amino acid sequence	Cannabis sativa

Synonyms

Synonyms	Comment	Organism
DELTA1-tetrahydrocannabinolic acid synthase	-	Cannabis sativa
THCA synthase	-	Cannabis sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.043	-	cannabigerolate	mutant enzyme R108A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.075	-	cannabigerolate	mutant enzyme R110A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.3	-	cannabigerolate	wild type enzyme, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa

Cofactor

Cofactor	Comment	Organism	Structure
FAD	the enzyme contains covalently attached FAD cofactor at a molar ratio of FAD to protein of 1:1	Cannabis sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.096	-	cannabigerolate	mutant enzyme R108A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.096	-	cannabigerolate	mutant enzyme R110A, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa
0.556	-	cannabigerolate	wild type enzyme, in 100 mM sodium citrate buffer (pH 5.0), at 30°C	Cannabis sativa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)