Literature summary for 1.21.3.7 extracted from

Shoyama, Y.; Takeuchi, A.; Taura, F.; Tamada, T.; Adachi, M.; Kuroki, R.; Shoyama, Y.; Morimoto, S.
Crystallization of Delta1-tetrahydrocannabinolic acid (THCA) synthase from Cannabis sativa (2005), Acta Crystallogr. Sect. F, 61, 799-801.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 insect cells	Cannabis sativa

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, in 0.09 M HEPES buffer pH 7.5 containing 1.26 M sodium citrate	Cannabis sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58600	-	x * 58600, calculated from amino acid sequence	Cannabis sativa
60000	-	x * 60000, SDS-PAGE	Cannabis sativa

Organism

Organism	UniProt	Comment	Textmining
Cannabis sativa	Q8GTB6	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the enzyme possesses at least eight potential N-linked glycosylation sites	Cannabis sativa

Purification (Commentary)

Purification (Comment)	Organism
Toyopearl CM-650 column chromatography, gel filtration	Cannabis sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cannabigerolate + O2	-	Cannabis sativa	DELTA9-tetrahydrocannabinolate + H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Cannabis sativa
?	x * 58600, calculated from amino acid sequence	Cannabis sativa

Synonyms

Synonyms	Comment	Organism
DELTA1-tetrahydrocannabinolic acid synthase	-	Cannabis sativa
THCA synthase	-	Cannabis sativa

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Release 2023.1 (January 2023)