Cloned (Comment) | Organism |
---|---|
for expression in Pichia pastoris cells | Eschscholzia californica |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of Berberine bridge enzyme in complex with dehydroscoulerine is determined to 1.63 A resolution | Eschscholzia californica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(S)-reticuline | - |
Eschscholzia californica | |
0.035 | - |
(S)-scoulerine | - |
Eschscholzia californica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + O2 | Eschscholzia californica | - |
(S)-scoulerine + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eschscholzia californica | P30986 | - |
- |
Purification (Comment) | Organism |
---|---|
in a two step purification process | Eschscholzia californica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-reticuline + O2 | - |
Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? | |
(S)-reticuline + O2 | (S)-scoulerine is further oxidized to dehydroscoulerine in a second oxidation step | Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(S)-reticuline oxidase | - |
Eschscholzia californica |
BBE | - |
Eschscholzia californica |
berberine bridge enzyme | - |
Eschscholzia californica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Eschscholzia californica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
(S)-scoulerine | - |
Eschscholzia californica | |
8 | - |
(S)-reticuline | - |
Eschscholzia californica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
activity assay | Eschscholzia californica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Eschscholzia californica |