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Literature summary for 1.21.3.3 extracted from

  • Winkler, A.; Puhl, M.; Weber, H.; Kutchan, T.M.; Gruber, K.; Macheroux, P.
    Berberine bridge enzyme catalyzes the six electron oxidation of (S)-reticuline to dehydroscoulerine (2009), Phytochemistry, 70, 1092-1097.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Pichia pastoris cells Eschscholzia californica

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of Berberine bridge enzyme in complex with dehydroscoulerine is determined to 1.63 A resolution Eschscholzia californica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
(S)-reticuline
-
Eschscholzia californica
0.035
-
(S)-scoulerine
-
Eschscholzia californica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-reticuline + O2 Eschscholzia californica
-
(S)-scoulerine + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Eschscholzia californica P30986
-
-

Purification (Commentary)

Purification (Comment) Organism
in a two step purification process Eschscholzia californica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-reticuline + O2
-
Eschscholzia californica (S)-scoulerine + H2O2
-
?
(S)-reticuline + O2 (S)-scoulerine is further oxidized to dehydroscoulerine in a second oxidation step Eschscholzia californica (S)-scoulerine + H2O2
-
?

Synonyms

Synonyms Comment Organism
(S)-reticuline oxidase
-
Eschscholzia californica
BBE
-
Eschscholzia californica
berberine bridge enzyme
-
Eschscholzia californica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Eschscholzia californica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
(S)-scoulerine
-
Eschscholzia californica
8
-
(S)-reticuline
-
Eschscholzia californica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
activity assay Eschscholzia californica

Cofactor

Cofactor Comment Organism Structure
FAD
-
Eschscholzia californica