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Literature summary for 1.21.3.3 extracted from

  • Winkler, A.; Lyskowski, A.; Riedl, S.; Puhl, M.; Kutchan, T.M.; Macheroux, P.; Gruber, K.
    A concerted mechanism for berberine bridge enzyme (2008), Nat. Chem. Biol., 4, 739-741.
    View publication on PubMed
Search for more literature for 1.21.3.3

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structures of berberine bridge enzyme in two different crystal forms, monoclinic and tetragonal, and in complex with (S)-reticuline are determined Eschscholzia californica

Protein Variants

Protein Variants Comment Organism
E417Q mutant, based on structural information, Glu417 essential amino acid for substrate oxidation, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica
H459A mutant, based on structural information, His459 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica
Y106F mutant, based on structural information, Tyr106 do not directly interact with the substrate, bicovalent flavin linkage is not affected by the mutation Eschscholzia californica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Eschscholzia californica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
BBE
-
 Eschscholzia californica
berberine bridge enzyme
-
 Eschscholzia californica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.054
-
 (S)-reticuline mutant E417Q Eschscholzia californica
0.7
-
 (S)-reticuline mutant Y106F Eschscholzia californica
3.1
-
 (S)-reticuline mutant H459A Eschscholzia californica
8
-
 (S)-reticuline wild-type enzyme Eschscholzia californica

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Eschscholzia californica