Literature summary for 1.20.99.1 extracted from

Duan, G.L.; Zhou, Y.; Tong, Y.P.; Mukhopadhyay, R.; Rosen, B.P.; Zhu, Y.G.
A CDC25 homologue from rice functions as an arsenate reductase (2007), New Phytol., 174, 311-321.

Search for more literature for 1.20.99.1

Activating Compound

Activating Compound	Comment	Organism	Structure
arsenate	-	Oryza sativa
arsenate	dramatically increases expression of ACR2.1	Oryza sativa
additional information	ACR2.1 exhibits higher reductase activity than ACR2.2	Oryza sativa
additional information	ACR2.1 exhibits higher reductase activity than ACR2.2. Expression of ACR2.1 under conditions of phosphate starvation is faster than in the presence of phosphate	Oryza sativa

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in an Escherichia coli strain in which the arsC gene is deleted and in a yeast (Saccharomyces cerevisiae) strain with a disrupted ACR2 gene	Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
C71S	mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities	Oryza sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	reduced glutaredoxin	-	Oryza sativa
0.2	-	reduced glutaredoxin	-	Oryza sativa
9.2	-	arsenate	-	Oryza sativa
9.9	-	arsenate	mutant C71S	Oryza sativa
12.2	-	arsenate	-	Oryza sativa
24.4	-	arsenate	mutant C71S	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14330	-	sequence analysis	Oryza sativa
14960	-	sequence analysis	Oryza sativa

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q10SX6	-	-
Oryza sativa	Q336V5	-	-

Purification (Commentary)

Purification (Comment)	Organism
by nickel-nitrilotriacetic acid chromatography	Oryza sativa

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	ACR2.2 is observed only in roots following arsenate exposure	Oryza sativa	-
root	weak expression of ACR2.1 before addition of arsenate	Oryza sativa	-
shoot	-	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + reduced glutaredoxin	yeast glutaredoxin ScGrx1p or Escherichia coli Grx2	Oryza sativa	arsenite + acceptor	-	?

Synonyms

Synonyms	Comment	Organism
ACR2.1	-	Oryza sativa
ACR2.2	-	Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	arsenate	mutant C70S	Oryza sativa
0.05	-	arsenate	mutant C71S	Oryza sativa
0.14	-	arsenate	-	Oryza sativa
0.19	-	reduced glutaredoxin	-	Oryza sativa
0.26	-	reduced glutaredoxin	-	Oryza sativa
0.33	-	arsenate	-	Oryza sativa

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Release 2023.1 (January 2023)