Crystallization (Comment) | Organism |
---|---|
free enzyme or enzyme complexed with arsenate and arsenite, X-ray diffraction structure determination and analysis at 1.65 A and 1.26 A, respecively | Escherichia coli |
in complex with arsenitate and arsenite and enzyme alone. Native structure shows sulfate and sulfite ions binding in the active site as analogs of arsenate and arsenite. Arsenate forms a covlaent adduct with C12 in the active site, showing tetrahedral geometry. The corresponding adduct with arsenite binds as thiarsahydroxy adduct. High number of 385 water molecules bound to crystal structure | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
sulfate | binds at the active site as arsenate analogue | Escherichia coli | |
sulfite | binds at the active site as arsenite analogue | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arsenate + reduced acceptor | Escherichia coli | detoxification enzyme, arsenite is extruded | arsenite + acceptor | - |
? | |
additional information | Escherichia coli | the arsRDABC plasmid operon codes for an ATP-dependent anion pump and is responsible for resistance against arsenate, arsenite, antimonite, and tellurite | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene arsC, encoded on plasmid R773 | - |
Escherichia coli | - |
bearing plasmid R773 conferring resistance to arsenite, arsenate, antimonite, and tellurite | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
arsenite + acceptor + H2O = arsenate + reduced acceptor | reaction mechanism, the catalytic Cys12, located at the active site, forms a covalent adduct with arsenate, the complex shows tetrahedral geometry, arsenite binds to Cys12 in a thiarsahydroxy adduct | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arsenate + reduced acceptor | - |
Escherichia coli | arsenite + acceptor | - |
? | |
arsenate + reduced acceptor | detoxification enzyme, arsenite is extruded | Escherichia coli | arsenite + acceptor | - |
? | |
additional information | the arsRDABC plasmid operon codes for an ATP-dependent anion pump and is responsible for resistance against arsenate, arsenite, antimonite, and tellurite | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ArsC | - |
Escherichia coli |
arsenate reductase | - |
Escherichia coli |