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Literature summary for 1.20.1.1 extracted from

  • Hirota, R.; Yamane, S.T.; Fujibuchi, T.; Motomura, K.; Ishida, T.; Ikeda, T.; Kuroda, A.
    Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506 (2012), J. Biosci. Bioeng., 113, 445-450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas stutzeri
expression in Escherichia coli Ralstonia sp.

Inhibitors

Inhibitors Comment Organism Structure
arsenite 4 mM, 90% residual activity Pseudomonas stutzeri
additional information not inhibitory: arsenite, NaCl Ralstonia sp.
NaCl 4 mM, 87% residual activity Pseudomonas stutzeri
nitrate 4 mM, 21% residual activity Pseudomonas stutzeri
nitrate 4 mM, 40% residual activity Ralstonia sp.
sulfite 4 mM, 16% residual activity Pseudomonas stutzeri
sulfite 4 mM, 56% residual activity Ralstonia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
NAD+ pH 7.2, 30°C Ralstonia sp.
0.0073
-
phosphonate pH 7.2, 30°C Pseudomonas stutzeri
0.0087
-
phosphonate pH 7.2, 30°C Ralstonia sp.
0.016
-
NAD+ pH 7.2, 40°C Ralstonia sp.
0.024
-
phosphonate pH 7.2, 40°C Ralstonia sp.
0.056
-
NAD+ pH 7.2, 30°C Pseudomonas stutzeri
0.128
-
NAD+ pH 7.2, 40°C Pseudomonas stutzeri
0.287
-
phosphonate pH 7.2, 40°C Pseudomonas stutzeri

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant protein Ralstonia sp.
-
-

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri WM88
-
-
-
Ralstonia sp. G4XDR8
-
-
Ralstonia sp. 4506 G4XDR8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphonate + NAD+ + H2O
-
Pseudomonas stutzeri phosphate + NADH + H+
-
?
phosphonate + NAD+ + H2O
-
Ralstonia sp. phosphate + NADH + H+
-
?
phosphonate + NAD+ + H2O
-
Pseudomonas stutzeri WM88 phosphate + NADH + H+
-
?
phosphonate + NAD+ + H2O
-
Ralstonia sp. 4506 phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
PtxD
-
Pseudomonas stutzeri
PtxD
-
Ralstonia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas stutzeri
50
-
-
Ralstonia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
half-life 80.5 h Pseudomonas stutzeri
45
-
half-life 80.5 h Ralstonia sp.