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Literature summary for 1.2.99.6 extracted from

  • White, H.; Feicht, R.; Huber, C.; Lottspeich, F.; Simon, H.
    Purification and some properties of the tungsten-containing carboxylic acid reductase from Clostridium formicoaceticum (1991), Biol. Chem. Hoppe-Seyler, 372, 999-1005.
    View publication on PubMed
Search for more literature for 1.2.99.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 Butanoate
-
 Clostridium formicaceticum
1.2
-
 4-Chlorobenzoate
-
 Clostridium formicaceticum
1.4
-
 4-Fluorobenzoate
-
 Clostridium formicaceticum
2.1
-
 4-Methoxybenzoate
-
 Clostridium formicaceticum
3.8
-
 3-chlorobenzoate
-
 Clostridium formicaceticum
5
-
 4-Methylbenzoate
-
 Clostridium formicaceticum
6.3
-
 propanoate 3-fluorobenzoate Clostridium formicaceticum
6.4
-
 propanoate
-
 Clostridium formicaceticum
12.8
-
 3-methylbenzoate
-
 Clostridium formicaceticum
16.7
-
 benzoate
-
 Clostridium formicaceticum
44.9
-
 3-Methoxybenzoate
-
 Clostridium formicaceticum
50.4
-
 3-hydroxybenzoate
-
 Clostridium formicaceticum
382
-
 4-hydroxybenzoate
-
 Clostridium formicaceticum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 11 mol of iron per mol of enzyme Clostridium formicaceticum
Tungsten contains 1.4 mol of tungsten per mol of enzyme Clostridium formicaceticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Clostridium formicaceticum
125000
-
 gel filtration Clostridium formicaceticum

Organism

Organism UniProt Comment Textmining
Clostridium formicaceticum
-
-
-

Oxidation Stability

Oxidation Stability Organism
22% loss of activity after 5 min in air, at 4°C Clostridium formicaceticum

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium formicaceticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Clostridium formicaceticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
 ?
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
 ?
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
Benzaldehyde + carbamoyl methyl viologen only reverse reaction described Clostridium formicaceticum Benzoate + reduced carbamoyl methyl viologen
-
 ?
Butanal + NH2CO-methyl viologen only reverse reaction described Clostridium formicaceticum Butanoate + reduced NH2CO-methyl viologen
-
 ?
Propanal + benzyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced benzyl viologen
-
 ?
Propanal + methyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced methyl viologen
-
 ?
Propanal + NH2CO-methyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced carbamoyl methyl viologen
-
 ?
Propanal + reduced 1,1',2,2'-tetramethylviologen
-
 Clostridium formicaceticum Propanoate + 1,1',2,2'-tetramethylviologen
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 67000, SDS-PAGE Clostridium formicaceticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 aldehyde dehydrogenation Clostridium formicaceticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 60% of maximal activity at 30°C and 50°C, inactive at 60°C, aldehyde dehydrogenation Clostridium formicaceticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of propanoate or benzoate Clostridium formicaceticum
9
-
 butanal and NH2-CO-methyl viologen as substrates Clostridium formicaceticum

pH Range

pH Minimum pH Maximum Comment Organism
6 7 pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate Clostridium formicaceticum
8 10 about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates Clostridium formicaceticum

Cofactor

Cofactor Comment Organism Structure
pterin contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme Clostridium formicaceticum