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Literature summary for 1.2.99.6 extracted from

  • Mukund, S.; Adams, M.W.W.
    The novel tungsten-iron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase. Evidence for its participation in a unique glycolytic pathway (1991), J. Biol. Chem., 266, 14208-14216.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
crotonaldehyde above 0.2 mM Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
crotonaldehyde
-
Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 7 gatom of Fe per mol of enzyme Pyrococcus furiosus
Tungsten contains 1 gatom of tungsten per mol of enzyme Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus its physiological role is proposed as the oxidation of glyceraldehyde to glycerate in a unique, partially nonphosphorylated glycolytic pathway that generates acetyl-CoA from glucose without participation of nicotinamide nucleotides ?
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Oxidation Stability

Oxidation Stability Organism
90% loss of activity after 10 min exposure to air Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53.6
-
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetaldehyde + methyl viologen
-
Pyrococcus furiosus acetate + reduced methyl viologen
-
?
Butanal + methyl viologen
-
Pyrococcus furiosus Butanoate + reduced methyl viologen
-
?
Crotonaldehyde + methyl viologen
-
Pyrococcus furiosus Crotonate + reduced methyl viologen
-
?
Glyceraldehyde + methyl viologen
-
Pyrococcus furiosus Glycerate + reduced methyl viologen
-
?
Methanal + methyl viologen
-
Pyrococcus furiosus Formate + reduced carbamoyl methyl viologen
-
?
additional information its physiological role is proposed as the oxidation of glyceraldehyde to glycerate in a unique, partially nonphosphorylated glycolytic pathway that generates acetyl-CoA from glucose without participation of nicotinamide nucleotides Pyrococcus furiosus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
above Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
activity increases up to pH 11 Pyrococcus furiosus