Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | incubation of the enzyme (14 mg/ml) in 100 mM Tris/HCl (pH 8.0) buffer with excess sodium sulfide (20 mM) under anaerobic conditions leads to an enhancement of the specific activity by 40% | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
oxidized ferredoxin | pH 8.4, 80°C | Pyrococcus furiosus | |
0.021 | - |
formaldehyde | pH 8.4, 80°C, with ferredoxin as electron acceptor | Pyrococcus furiosus | |
0.025 | - |
formaldehyde | pH 8.4, 20°C | Pyrococcus furiosus | |
0.033 | - |
formaldehyde | pH 8.4, 80°C | Pyrococcus furiosus | |
0.071 | - |
formaldehyde | pH 8.4, 80°C, with benzyl viologen as electron acceptor | Pyrococcus furiosus | |
0.073 | - |
Oxidized benzyl viologen | pH 8.4, 80°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains one [4Fe-4S] cubane per 69000 Da subunit | Pyrococcus furiosus | |
Tungsten | contains one tungstodipterin per 69000 Da subunit | Pyrococcus furiosus | |
Tungsten | the tungsten in the active enzyme is a two-electron acceptor, W(VI/IV) | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Pyrococcus furiosus | Q8U1K3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + H2O + 2 oxidized ferredoxin | - |
Pyrococcus furiosus | formate + 2 H+ + 2 reduced ferredoxin | - |
? | |
formaldehyde + H2O + oxidized benzyl viologen | - |
Pyrococcus furiosus | formate + H+ + reduced benzyl viologen | - |
? | |
formaldehyde + H2O + oxidized benzyl viologen | no activity with methanol or formic acid as substrate | Pyrococcus furiosus | formate + 2 H+ + reduced benzyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
formaldehyde ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
formaldehyde | pH 8.4, 20°C | Pyrococcus furiosus | |
54 | - |
formaldehyde | pH 8.4, 80°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
560 | - |
formaldehyde | pH 8.4, 20°C | Pyrococcus furiosus | |
1636 | - |
formaldehyde | pH 8.4, 80°C | Pyrococcus furiosus |