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Literature summary for 1.2.7.8 extracted from

  • Schut, G.J.; Menon, A.L.; Adams, M.W.W.
    2-Keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis (2001), Methods Enzymol., 331, 144-158.
    View publication on PubMed
Search for more literature for 1.2.7.8

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate
-
 Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 CoA
-
 Pyrococcus furiosus
0.048
-
 Ferredoxin
-
 Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
24000
-
 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
66000
-
 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
71000
-
 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin Methanothermobacter thermautotrophicus
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin Pyrococcus furiosus
-
 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Pyrococcus furiosus
-
-
-

Oxidation Stability

Oxidation Stability Organism
irreversibly inactivated by oxygen Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Methanothermobacter thermautotrophicus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin
-
 r
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+
-
 r
2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA
-
 r
2-ketoisocaproate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus CO2 + reduced ferredoxin + isovaleryl-CoA
-
 r
indolepyruvate + CoA + oxidized methylviologen
-
 Pyrococcus furiosus indoleacetyl-CoA + CO2 + reduced methylviologen
-
 ?
additional information pyruvate, 2-ketoisovalerate, 2-ketoglutarate, 2 ketobutyrate, and phenylglyoxylate are no substrates Pyrococcus furiosus ?
-
 ?
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
phenylpyruvate + CoA + oxidized ferredoxin
-
 Methanothermobacter thermautotrophicus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r
phenylpyruvate + CoA + oxidized ferredoxin
-
 Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
 r

Subunits

Subunits Comment Organism
tetramer
-
 Methanothermobacter thermautotrophicus
tetramer 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus
tetramer 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE Pyrococcus furiosus