Application | Comment | Organism |
---|---|---|
synthesis | engineering of Clostridium thermocellum to produce isobutanol. Both the native 2-oxoisovalerate-oxidoreductase KOR, EC 1.2.7.7, and the heterologous Lactococcus lactis 2-oxoisovalerate decarboxylase KIVD, EC 4.1.1.74, expressed are responsible for isobutanol production.The plasmid is integrated into the chromosome by single crossover. The resulting strain is stable without antibiotic selection pressure and produces 5.4g/l of isobutanol from cellulose in minimal medium at 50°C within 75 h, corresponding to 41% of theoretical yield | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
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Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus DSM 1313 | - |
- |
- |