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Literature summary for 1.2.7.6 extracted from

  • Roy, R.; Menon, A.L.; Adams, M.W.W.
    Aldehyde oxidoreductases from Pyrococcus furiosus (2001), Methods Enzymol., 331, 132-144.
    View publication on PubMed
Search for more literature for 1.2.7.6

Activating Compound

Activating Compound Comment Organism Structure
Tungsten stimulates cell growth when added to the medium Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 D-Glyceraldehyde-3-phosphate with ferredoxin as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 [4Fe-4S]2+ cluster per enzyme molecule Pyrococcus furiosus
Fe2+ 5-6 iron g-atoms per mol of enzyme Pyrococcus furiosus
Mg2+ contains 1 molecule per enzyme Pyrococcus furiosus
Tungsten 1 tungsten center per molecule of enzyme Pyrococcus furiosus
Zn2+ contains 2 molecules per enzyme Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive, t1/2 at 23°C: 6 h Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 strictly anaerobic assay conditions Pyrococcus furiosus
25
-
 purified enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen artificial electron acceptor Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?
additional information no activity with NAD(P)+ as electron acceptor Pyrococcus furiosus ?
-
 ?
additional information no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 73000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 t1/2: 15 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Pyrococcus furiosus
tungsten-molybdopterin tungstopterin center Pyrococcus furiosus
tungsten-molybdopterin structure Pyrococcus furiosus