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Literature summary for 1.2.7.5 extracted from

  • Roy, R.; Menon, A.L.; Adams, M.W.W.
    Aldehyde oxidoreductases from Pyrococcus furiosus (2001), Methods Enzymol., 331, 132-144.
    View publication on PubMed
Search for more literature for 1.2.7.5

Activating Compound

Activating Compound Comment Organism Structure
Sulfide activation of formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Sulfide 4-5fold increase in activity at room temperature and pH 8.0 at 20 mM, in presence of 20 mM dithionite Pyrococcus furiosus
Tungsten stimulates cell growth Pyrococcus furiosus
Tungsten stimulates cell growth Thermococcus litoralis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.028
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.05
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.057
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.076
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.14
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.15
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
0.8
-
 glutaric dialdehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.42
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
8
-
 Succinic semialdehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
15
-
 phenylpropionaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
25
-
 formaldehyde formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor Pyrococcus furiosus
25
-
 Indoleacetaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
60
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Pyrococcus furiosus
60
-
 propionaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 Ca2+ per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus sp.
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus litoralis
Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus endeavori
Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori
Fe2+ contains 1 additional Fe2+ besides the [4Fe-4S]-cluster Pyrococcus furiosus
Mg2+ both enzyme forms Pyrococcus furiosus
Mg2+ contains 1 Mg-atom per subunit Pyrococcus furiosus
Mg2+ contains 1 Mg-atom per subunit Thermococcus sp.
Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori
Tungsten 1 tungsten per subunit Pyrococcus furiosus
Tungsten 1 tungsten per subunit Thermococcus sp.
Tungsten 1 tungsten per subunit Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
67000
-
 2 * 67000 Pyrococcus furiosus
136100
-
-
 Pyrococcus furiosus
280000
-
-
 Pyrococcus furiosus
280000
-
-
 Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an aldehyde + H2O + oxidized ferredoxin Thermococcus sp.
-
 an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin Pyrococcus endeavori
-
 an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin Thermococcus litoralis formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin Thermococcus sp. ES1
-
 an acid + H+ + reduced ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus endeavori
-
 hyperthermophilic archaeon
-
Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
Pyrococcus furiosus
-
 strain DSM 3638
-
Pyrococcus furiosus
-
 contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
-
Pyrococcus furiosus Q51739 hyperthermophilic archaeon
-
Pyrococcus furiosus Q51739 strain DSM 3638
-
Pyrococcus furiosus Q51739 gene aor
-
Thermococcus litoralis
-
 hyperthermophilic archaeon
-
Thermococcus litoralis
-
 formaldehyde ferredoxin oxidoreductase
-
Thermococcus sp.
-
 hyperthermophilic archaeon
-
Thermococcus sp. ES1
-
 hyperthermophilic archaeon
-

Oxidation Stability

Oxidation Stability Organism
aldehyde ferredoxin oxidoreductase, t1/2: 30 min at 23 °C Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, t1/2: 9 h at 23°C Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
formaldehyde and aldehyde ferredoxin oxidoreductase Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required Pyrococcus furiosus
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. acetate + H+ + reduced benzyl viologen
-
 ir
acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced benzyl viologen
-
 ir
acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis acetate + H+ + reduced benzyl viologen
-
 ir
acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 acetate + H+ + reduced benzyl viologen
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus litoralis an acid + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus endeavori an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus endeavori an acid + H+ + reduced ferredoxin
-
 ir
an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea Thermococcus litoralis an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. ES1 an acid + H+ + reduced ferredoxin
-
 ?
an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. ES1 an acid + H+ + reduced ferredoxin
-
 ir
benzaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. benzoic acid + H+ + reduced benzyl viologen
-
 ir
benzaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis benzoic acid + H+ + reduced benzyl viologen
-
 ?
crotonaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus crotonate + H+ + reduced benzyl viologen
-
 ir
crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. crotonate + H+ + reduced benzyl viologen
-
 ir
crotonaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis crotonate + H+ + reduced benzyl viologen
-
 ?
crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 crotonate + H+ + reduced benzyl viologen
-
 ir
formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. formate + H+ + reduced benzyl viologen
-
 ir
formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ir
formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis formate + H+ + reduced benzyl viologen
-
 ir
formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 formate + H+ + reduced benzyl viologen
-
 ir
glutaraldehyde + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus glutarate + H+ + reduced methyl viologen
-
 ?
glutaric dialdehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis ?
-
 ir
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus indoleacetate + H+ + reduced benzyl viologen
-
 ?
indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. indoleacetate + H+ + reduced benzyl viologen
-
 ir
indoleacetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis indoleacetate + H+ + reduced benzyl viologen
-
 ir
isovalerylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. isovalerate + H+ + reduced benzyl viologen
-
 ir
isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Pyrococcus furiosus isovalerate + H+ + reduced benzyl viologen
-
 ?
isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Thermococcus litoralis isovalerate + H+ + reduced benzyl viologen
-
 ?
phenylacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. phenylacetate + H+ + reduced benzyl viologen
-
 ir
phenylacetaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis phenylacetate + H+ + reduced benzyl viologen
-
 ?
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus phenylpropanoate + H+ + reduced benzyl viologen
-
 r
phenylpropionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis phenylpropanoate + H+ + reduced benzyl viologen
-
 ir
propionaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. propionate + H+ + reduced benzyl viologen
-
 ir
propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced benzyl viologen
-
 ir
propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis propionate + H+ + reduced benzyl viologen
-
 ir
succinic semialdehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus succinic acid + H+ + reduced benzyl viologen
-
 r
succinic semialdehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis succinic acid + H+ + reduced benzyl viologen
-
 ir

Subunits

Subunits Comment Organism
dimer
-
 Pyrococcus furiosus
dimer 2 * 67000, SDS-PAGE Thermococcus sp.
dimer 2 * 67000 Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Pyrococcus furiosus
80
-
 assay at Thermococcus sp.
80
-
 assay at Thermococcus litoralis
80
-
 assay at Pyrococcus endeavori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 formaldehyde ferredoxin oxidoreductase, t1/2: 6 h Pyrococcus furiosus
80
-
 aldehyde ferredoxin oxidoreductase, t1/2: 15 min Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Pyrococcus furiosus
8.4
-
 assay at Thermococcus sp.
8.4
-
 assay at Thermococcus litoralis
8.4
-
 assay at Pyrococcus endeavori

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Pyrococcus furiosus
Ferredoxin
-
 Thermococcus sp.
Ferredoxin
-
 Thermococcus litoralis
Ferredoxin
-
 Pyrococcus endeavori
tungsten cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
tungsten cofactor formaldehyde ferredoxin oxidoreductase Thermococcus litoralis
tungsten cofactor pterin ring system Pyrococcus furiosus
tungsten cofactor pterin ring system Thermococcus sp.
tungsten cofactor pterin ring system Thermococcus litoralis
tungsten cofactor pterin ring system Pyrococcus endeavori
tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori