Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfide | activation of formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus |
General Stability | Organism |
---|---|
10% glycerol and 2 mM DTT stabilize during purification | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | formaldehyde ferredoxin oxidoreductase, Km for several substrates at different concentrations with purified and sulfide-activated enzyme, overview | Pyrococcus furiosus | |
25 | - |
formaldehyde | formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.4 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Fe2+ | 3.8 gatoms per subunit, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Mg2+ | 1.5 mol per mol of subunit, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | |
Tungsten | 0.9 gatom per subunit, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE | Pyrococcus furiosus |
275000 | - |
formaldehyde ferredoxin oxidoreductase, gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aldehyde + H2O + oxidized ferredoxin | Pyrococcus furiosus | - |
an acid + H+ + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
hyperthermophilic archaeon | - |
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Pyrococcus furiosus | - |
contains a formaldehyde and an aldehyde ferredoxin oxidoreductase | - |
Pyrococcus furiosus | - |
genes aor and for | - |
Purification (Comment) | Organism |
---|---|
formaldehyde ferredoxin oxidoreductase, 17fold | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
formaldehyde ferredoxin oxidoreductase, substrate specificity | Pyrococcus furiosus |
42 | - |
purified formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | acetate + H+ + reduced benzyl viologen | - |
ir | |
acetaldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | acetate + H+ + reduced ferredoxin | - |
ir | |
an aldehyde + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
? | |
an aldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase, C1-C3 aldehydes | Pyrococcus furiosus | an acid + H+ + reduced ferredoxin | - |
ir | |
butyraldehyde + H2O + oxidized ferredoxin | low activity | Pyrococcus furiosus | butyrate + H+ + reduced ferredoxin | - |
ir | |
butyraldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | butyrate + H+ + reduced ferredoxin | - |
ir | |
crotonaldehyde + H2O + oxidized benzyl viologen | low activity, formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | crotonate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | formate + H+ + reduced benzyl viologen | - |
ir | |
formaldehyde + H2O + oxidized ferredoxin | formaldehyde and aldehyde ferredoxin oxidoreductases | Pyrococcus furiosus | formate + H+ + reduced ferredoxin | - |
ir | |
isovalerylaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase, no activity | Pyrococcus furiosus | isovalerate + H+ + reduced benzyl viologen | - |
? | |
propionaldehyde + H2O + oxidized benzyl viologen | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | propionate + H+ + reduced benzyl viologen | - |
ir | |
propionaldehyde + H2O + oxidized ferredoxin | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus | propionate + H+ + reduced ferredoxin | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, formaldehyde ferredoxin oxidoreductase, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
FOR | - |
Pyrococcus furiosus |
More | formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | formaldehyde ferredoxin oxidoreductase, activity increases 4.5fold from 60°C to 90°C at pH 8.4 | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermostable | Pyrococcus furiosus |
80 | - |
formaldehyde ferredoxin oxidoreductase, t1/2: 8 h, in presence of 2 mM dithionite and 2 mM DTT, pH 8.4 | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | formaldehyde ferredoxin oxidoreductase, activity increases linearly from pH 5.5 to pH 10.0 at 80°C | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pyrococcus furiosus | |
form A cofactor | formaldehyde ferredoxin oxidoreductase | Pyrococcus furiosus |