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Literature summary for 1.2.7.4 extracted from

  • Soboh, B.; Linder, D.; Hedderich, R.
    Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans (2002), Eur. J. Biochem., 269, 5712-5721.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ti(III)citrate 1mM for 1 min at 65°C, 45fold increase in initial activity Carboxydothermus hydrogenoformans

Inhibitors

Inhibitors Comment Organism Structure
CO 45% CO in headspace, 50% inhibition Carboxydothermus hydrogenoformans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Carboxydothermus hydrogenoformans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 61.5 mol nonheme iron per mol of complex Carboxydothermus hydrogenoformans
Nickel 3 mol per mol of complex Carboxydothermus hydrogenoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits Carboxydothermus hydrogenoformans
62000
-
2 * 62000, SDS-PAGE, enzyme Carboxydothermus hydrogenoformans
120000
-
gel filtration, enzyme Carboxydothermus hydrogenoformans
450000
-
gel filtration, complex Carboxydothermus hydrogenoformans

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans
-
enzyme CooS in complex with an electron transfer protein CooF and a membrane bound [Ni-Fe] hydrogenase
-

Purification (Commentary)

Purification (Comment) Organism
-
Carboxydothermus hydrogenoformans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5924
-
pH 7.0, 70°C Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + methyl viologen optimal concentration of CO 5% in headspace Carboxydothermus hydrogenoformans CO2 + reduced methyl viologen
-
?
H2 + reduced methyl viologen
-
Carboxydothermus hydrogenoformans methyl viologen
-
r

Subunits

Subunits Comment Organism
dimer 2 * 62000, SDS-PAGE, enzyme Carboxydothermus hydrogenoformans
octamer 2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits Carboxydothermus hydrogenoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
both for enzyme and complex Carboxydothermus hydrogenoformans