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Literature summary for 1.2.7.4 extracted from

  • Bonam, D.; Ludden, P.W.
    Purification and characterization of carbon monoxide dehydrogenase, a nickel, zinc, iron-sulfur protein, from Rhodospirillum rubrum (1987), J. Biol. Chem., 262, 2980-2987.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme form I contains 7 iron and 6 sulfur per monomer Rhodospirillum rubrum
Iron enzyme form II contains 9 iron and 8 sulfur per monomer Rhodospirillum rubrum
Nickel enzyme form I contains 0.6 mol of nickel per mol of monomer Rhodospirillum rubrum
Nickel enzyme form II contains 1.4 mol of nickel per mol of monomer Rhodospirillum rubrum
Zinc enzyme form I contains 0.4 mol of zinc per mol of monomer Rhodospirillum rubrum
Zinc enzyme form II contains 0.8 mol of zinc per mol of monomer Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61600
-
gel filtration Rhodospirillum rubrum
61800
-
1 * 61800, SDS-PAGE Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
purple nonsulfur bacterium, the enzyme exists in the forms, I and II, which differ in metal content
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodospirillum rubrum

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ammonium malate-grown cell induced with 100% CO 24 h before harvesting Rhodospirillum rubrum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
694
-
-
Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO + H2O + methyl viologen
-
Rhodospirillum rubrum CO2 + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
monomer 1 * 61800, SDS-PAGE Rhodospirillum rubrum