Cloned (Comment) | Organism |
---|---|
expression of forDABGE in Escherichia coli JM109, structure of gene cluster | Hydrogenobacter thermophilus |
expression of korAB in Escherichia coli JM109, structure of gene cluster | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
CoA | pH 7.8, 70°C, recombinant For | Hydrogenobacter thermophilus | |
0.08 | - |
CoA | pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus | |
1.4 | - |
2-oxoglutarate | pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus | |
2.9 | - |
2-oxoglutarate | pH 7.8, 70°C, recombinant For | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
recombinant For, gel filtration | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For | - |
Hydrogenobacter thermophilus | Q9AJM0 and Q9AJL9 | Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE; two distinct OGOR enzymes termed Kor and For | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | Q9AJM0 and Q9AJL9 | Q9AJM0: subunit korA, Q9AJL9: subunit korB; two distinct OGOR enzymes termed Kor and For | - |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of For is approximately one-tenth of that of Kor | Hydrogenobacter thermophilus |
35.2 | - |
pH 7.8, 70°C, recombinant Kor | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | the For enzyme has a five-subunit-type structure, the molar ratio of ForA:ForB:ForG:ForD:ForE is 1.0:1.04:1.02:0.98:0.2, the sum of the calculated MWs of the five For subunits is 136 kDa | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
FOR enzyme | - |
Hydrogenobacter thermophilus |
KOR enzyme | - |
Hydrogenobacter thermophilus |
OGOR | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
recombinant For enzyme | Hydrogenobacter thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 35% of maximal activity, 90°C: about 50% of maximal activity, recombinant For, the recombinant For enzyme is inactive at room temperature | Hydrogenobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant Kor, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
80 | - |
recombinant For, about 70% loss of activity under aerobic conditions, no loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
80 | - |
recombinant Kor, about 95% loss of activity under aerobic conditions, about 25% loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
90 | - |
recombinant For, about 90% loss of activity under aerobic conditions, about 30% loss of activity under anaerobic conditions | Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
recombinant Kor | Hydrogenobacter thermophilus |
8.7 | - |
recombinant For, at 70°C, optimum varies between buffers | Hydrogenobacter thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.7 | pH 7.0: about 45% of maximal activity, pH 9.7: about 65% of maximal activity, recombinant For | Hydrogenobacter thermophilus |
Organism | Comment | Expression |
---|---|---|
Hydrogenobacter thermophilus | the For enzyme is expressed constitutively during oxygen respiration. Little expression of For is observed during cultivation under conditions of nitrate respiration | additional information |
Hydrogenobacter thermophilus | the Kor enzyme is constitutively expressed during respiration of either oxygen or nitrate respiration | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | the For enzyme supports the optimal growth of the organism in the presence of oxygen | Hydrogenobacter thermophilus |