General Stability | Organism |
---|---|
stabilized under anaerobic conditions by a combination of dimercaptoethanol and FeSO4 | Prosthecochloris vibrioformis f. thiosulfatophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
succinyl-CoA | pH and temperature not specified in the publication | Prosthecochloris vibrioformis f. thiosulfatophilum | |
2.6 | - |
2-oxoglutarate | pH and temperature not specified in the publication | Prosthecochloris vibrioformis f. thiosulfatophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of Mg2+ | Prosthecochloris vibrioformis f. thiosulfatophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
220000 | - |
sucrose density gradient sedimentation | Prosthecochloris vibrioformis f. thiosulfatophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prosthecochloris vibrioformis f. thiosulfatophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Prosthecochloris vibrioformis f. thiosulfatophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.25 | - |
pH and temperature not specified in the publication | Prosthecochloris vibrioformis f. thiosulfatophilum |
Storage Stability | Organism |
---|---|
0°C, anaerobic conditions, 2,3-dimercaptoethanol plus FeSO4 | Prosthecochloris vibrioformis f. thiosulfatophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | of several ferredoxins tested, only those from the photosynthetic bacteria Chlorobium thiosulfatophilum, Chromatium, and Rhodospirillum rubrum are effective. The ferredoxins of Clostridium pasteurianum and spinach are poor substitutes for those of photosynthetic bacteria | Prosthecochloris vibrioformis f. thiosulfatophilum | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | - |
r |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutarate synthase | - |
Prosthecochloris vibrioformis f. thiosulfatophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | optimum with a sharp drop on the acidic side | Prosthecochloris vibrioformis f. thiosulfatophilum |