Application | Comment | Organism |
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synthesis | engineering of Thermoanaerobacterium saccharolyticum to produce ethanol at high yield. Knockout of genes involved in organic acid formation (acetate kinase, phosphate acetyltransferase, and L-lactate dehydrogenase) results in a strain able to produce ethanol as the only detectable organic product and substantial changes in electron flow relative to the wild type. Ethanol formation in the engineered strain ALK2 utilizes pyruvate:ferredoxin oxidoreductase with electrons transferred from ferredoxin to NAD(P). The homoethanologenic phenotype is stable for more than 150 generations in continuous culture. The growth rate of strain ALK2 is similar to the wild-type strain, with a reduction in cell yield proportional to the decreased ATP availability resulting from acetate kinase inactivation. Glucose and xylose are coutilized and utilization of mannose and arabinose commences before glucose and xylose are exhausted. Using strain ALK2 in simultaneous hydrolysis and fermentation experiments at 50°C allows a 2.5fold reduction in cellulase loading compared with using Saccharomyces cerevisiae at 37°C. The maximum ethanol titer produced by strain ALK2 is 37 g/liter | Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
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Thermoanaerobacterium saccharolyticum | - |
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