Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus profundus | Q9HHD4 | ferredoxin | - |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | enzyme contains an extremely thermostable [4Fe-4S] ferredoxin. The Fe-S cluster has three cysteines and one aspartate as the cluster ligands. The 4Fe ferredoxin is degraded to 3Fe ferredoxin during aerobic purification. The aerobically-purified ferredoxin is reversibly converted back to the [4Fe-4S] ferredoxin by the addition of ferrous ions under reducing conditions. The anaerobically-purified [4Fe-4S] ferredoxin is quite stable, little degradtion is observed over 20 h at 100°C, while the half-life of the aerobically-purified ferredoxin is 10 h at 100°C | Thermococcus profundus |