Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.7.1 extracted from

  • Noth, J.; Krawietz, D.; Hemschemeier, A.; Happe, T.
    Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii (2013), J. Biol. Chem., 288, 4368-4377.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.7.1

Application

Application Comment Organism
biotechnology pyruvate:ferredoxin oxidoreductase PFR1 and [Fe-Fe]-hydrogenase HYDA1 of Chlamydomonas can be coupled for pyruvate-dependent H2 production Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 CoA in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication Chlamydomonas reinhardtii
1.65
-
 pyruvate in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication Chlamydomonas reinhardtii
2.33
-
 oxidized methyl viologen in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
144000
-
 x * 144000, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Chlamydomonas reinhardtii the enzyme cannot use 2-oxoglutarate as substrate ?
-
 ?
additional information Chlamydomonas reinhardtii the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers ?
-
 ?
additional information Chlamydomonas reinhardtii CC124 the enzyme cannot use 2-oxoglutarate as substrate ?
-
 ?
additional information Chlamydomonas reinhardtii CC124 the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers ?
-
 ?
pyruvate + CoA + oxidized ferredoxin Chlamydomonas reinhardtii
-
 acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
pyruvate + CoA + oxidized ferredoxin Chlamydomonas reinhardtii CC124
-
 acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii CC124
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cannot use 2-oxoglutarate as substrate Chlamydomonas reinhardtii ?
-
 ?
additional information the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers Chlamydomonas reinhardtii ?
-
 ?
additional information the enzyme cannot use 2-oxoglutarate as substrate Chlamydomonas reinhardtii CC124 ?
-
 ?
additional information the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers Chlamydomonas reinhardtii CC124 ?
-
 ?
oxaloacetate + CoA + oxidized methyl viologen
-
 Chlamydomonas reinhardtii ? + reduced methyl viologen + H+
-
 ?
oxaloacetate + CoA + oxidized methyl viologen
-
 Chlamydomonas reinhardtii CC124 ? + reduced methyl viologen + H+
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
pyruvate + CoA + oxidized ferredoxin
-
 Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced ferredoxin + H+
-
 ?
pyruvate + CoA + oxidized methyl viologen
-
 Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?
pyruvate + CoA + oxidized methyl viologen
-
 Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 144000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

Synonyms Comment Organism
PFOR
-
 Chlamydomonas reinhardtii
PFR1
-
 Chlamydomonas reinhardtii
pyruvate:ferredoxin oxidoreductase
-
 Chlamydomonas reinhardtii