Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | the purified enzyme is slightly stimulated by high phosphate concentrations. Addition of 200 mM potassium (or sodium) phosphate stimulates the enzyme activity 3-4fold | Methanosarcina barkeri |
General Stability | Organism |
---|---|
the enzyme is extremely oxygen sensitive, losing 90% of its activity upon exposure to air for 1 h at 0°C | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium nitrite | - |
Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
CoA | 37°C, pH 7.0 | Methanosarcina barkeri | |
0.03 | - |
oxidized ferredoxin | 37°C, pH 7.0, ferredoxin from Clostridium pasteurianum | Methanosarcina barkeri | |
0.07 | - |
pyruvate | 37°C, pH 7.0 | Methanosarcina barkeri | |
0.07 | - |
Oxidized benzyl viologen | 37°C, pH 7.0 | Methanosarcina barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains about 12 mol of non-heme iron per mol of enzyme | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 48000 + x * 30000 + x * 25000 + x * 15000, SDS-PAGE | Methanosarcina barkeri |
25000 | - |
x * 48000 + x * 30000 + x * 25000 + x * 15000, SDS-PAGE | Methanosarcina barkeri |
30000 | - |
x * 48000 + x * 30000 + x * 25000 + x * 15000, SDS-PAGE | Methanosarcina barkeri |
48000 | - |
x * 48000 + x * 30000 + x * 25000 + x * 15000, SDS-PAGE | Methanosarcina barkeri |
130000 | - |
gel filtration | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2 oxidized ferredoxin | Methanosarcina barkeri | first enzyme of pyruvate catabolism | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | Methanosarcina barkeri DSM 804 | first enzyme of pyruvate catabolism | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | P80521 and P80522 and P80523 and P80524 | P80521: subunit A, P80522: subunit B, P80523: subunit C, P80524: subunit D | - |
Methanosarcina barkeri DSM 804 | P80521 and P80522 and P80523 and P80524 | P80521: subunit A, P80522: subunit B, P80523: subunit C, P80524: subunit D | - |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Storage Stability | Organism |
---|---|
-20°C, under N2, after addition of 20% (v/v) glycerol to each fraction, the enzyme activity remains nearly constant for several weeks | Methanosarcina barkeri |
4°C, storage results in a loss of 80% of the activity within 8 days | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + CoA + 2 oxidized benzyl viologen | coenzyme F420 is not reduced by the purified enzyme | Methanosarcina barkeri | propanoyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
2-oxobutyrate + CoA + 2 oxidized benzyl viologen | coenzyme F420 is not reduced by the purified enzyme | Methanosarcina barkeri DSM 804 | propanoyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
additional information | the enzyme does not catalyze the oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, 3-hydroxypyruvate and oxaloacetate | Methanosarcina barkeri | ? | - |
? | |
additional information | the enzyme does not catalyze the oxidation of 2-oxoglutarate, indolepyruvate, phenylpyruvate, glyoxylate, 3-hydroxypyruvate and oxaloacetate | Methanosarcina barkeri DSM 804 | ? | - |
? | |
pyruvate + CoA + 2 oxidized benzyl viologen | coenzyme F420 is not reduced by the purified enzyme | Methanosarcina barkeri | acetyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
pyruvate + CoA + 2 oxidized benzyl viologen | coenzyme F420 is not reduced by the purified enzyme | Methanosarcina barkeri DSM 804 | acetyl-CoA + CO2 + 2 reduced benzyl viologen | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | first enzyme of pyruvate catabolism | Methanosarcina barkeri | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | ferredoxin from Clostridium pasteurianum | Methanosarcina barkeri | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | first enzyme of pyruvate catabolism | Methanosarcina barkeri DSM 804 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | ferredoxin from Clostridium pasteurianum | Methanosarcina barkeri DSM 804 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000 + x * 30000 + x * 25000 + x * 15000, SDS-PAGE | Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
59 | - |
- |
Methanosarcina barkeri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | 71 | 43°C: about 55% of maximal activity, 71°C: about 5% of maximal activity | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | contains 1 mol thiamine diphosphate per mol of enzyme | Methanosarcina barkeri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
Sodium nitrite | 37°C, pH 7.0 | Methanosarcina barkeri |
General Information | Comment | Organism |
---|---|---|
physiological function | first enzyme of pyruvate catabolism | Methanosarcina barkeri |