Literature summary for 1.2.5.3 extracted from

Lorite, M.J.; Tachil, J.; Sanjuan, J.; Meyer, O.; Bedmar, E.J.
Carbon monoxide dehydrogenase activity in Bradyrhizobium japonicum (2000), Appl. Environ. Microbiol., 66, 1871-1876.

Search for more literature for 1.2.5.3

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	yields di(carboxamidomethyl)molybdopterin cytosine dinucleotide from reaction with the molybdenum cofactor	Bradyrhizobium japonicum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Bradyrhizobium japonicum	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	7.6 mol/mol of enzyme	Bradyrhizobium japonicum
Mo2+	7.6 mol/mol of enzyme	Bradyrhizobium japonicum
additional information	the enzyme contains 2.29 mol of Mo, 7.96 mol of Fe, 7.60 mol of S, and 1.99 mol of flavins at a 1.15:4:3.82:1 molar ratio, but contains no tungsten	Bradyrhizobium japonicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17200	-	(alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE	Bradyrhizobium japonicum
28400	-	(alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE	Bradyrhizobium japonicum
75000	-	(alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE	Bradyrhizobium japonicum
230000	-	native PAGE	Bradyrhizobium japonicum

Organism

Organism	UniProt	Comment	Textmining
Bradyrhizobium japonicum	-	-	-
Bradyrhizobium japonicum 110spc4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 21fold from cell-free extract by ultracentrifugation, anion exchange chroomatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration	Bradyrhizobium japonicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:CO-grown cell	chemolithoautotrophic, CO-dependent growth of strain 110spc4, carbon monoxide as a sole energy and carbon source under aerobic conditions	Bradyrhizobium japonicum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.058	-	purified enzyme, pH 7.5, 30°C	Bradyrhizobium japonicum

Subunits

Subunits	Comment	Organism
heterohexamer	(alphabetagamma)2, 2 * 75000, large subunit, + 1 * 28400, medium subunit, + 1 * 17200, small subunit, SDS-PAGE	Bradyrhizobium japonicum

Synonyms

Synonyms	Comment	Organism
CODH	-	Bradyrhizobium japonicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bradyrhizobium japonicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bradyrhizobium japonicum

Cofactor

Cofactor	Comment	Organism	Structure
flavin	-	Bradyrhizobium japonicum
molybdenum cofactor	molybdopterin cytosine dinucleotide as the organic portion of the Bradyrhizobium japonicum CODH molybdenum cofactor	Bradyrhizobium japonicum
additional information	the enzyme contains 2.29 mol of Mo, 7.96 mol of Fe, 7.60 mol of S, and 1.99 mol of flavins at a 1.15:4:3.82:1 molar ratio, but contains no tungsten	Bradyrhizobium japonicum
phenazine methosulfate	artificial electron carrier	Bradyrhizobium japonicum

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Release 2023.1 (January 2023)