Crystallization (Comment) | Organism |
---|---|
crystals of E1b grown in the absence and presence of substrates at 22ΒΊC via the vapor-diffusion method, key tyrosine residue in the E1b active site, functions as a conformational switch to reduce the reactivity of the thiamin diphosphate cofactor, the tyrosine switch further remodels an E1b loop region to promote E1b binding to E2b | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | hE1b-Tyr113Phe mutant, specifically affects the decarboxylation reaction, thiamine diphosphate appears hyperactive, is prone to paracatalytic inactivation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-keto-beta-methylvaleric acid + NADH | Homo sapiens | - |
? | - |
? | |
alpha-ketoisocaproic acid + NADH | Homo sapiens | - |
? | - |
? | |
alpha-ketoisovaleric acid + NADH | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-keto-beta-methylvaleric acid + NADH | - |
Homo sapiens | ? | - |
? | |
alpha-ketoisocaproic acid + NADH | - |
Homo sapiens | ? | - |
? | |
alpha-ketoisovaleric acid + NADH | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCKDC | - |
Homo sapiens |
branched-chain alpha-ketoacid dehydrogenase complex | - |
Homo sapiens |
E1b | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1983 | - |
alpha-ketoisovaleric acid | wild-type | Homo sapiens | |
2.85 | - |
alpha-ketoisovaleric acid | hE1b-Tyr113Phe mutant | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Homo sapiens |