Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 12% (w/v) polyethylene glycol 4000, 50 mM sodium citrate (pH 5.6) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H260A | mutant has a dramatically reduced catalytic rate | Escherichia coli |
H298A | mutant has a dramatically reduced catalytic rate | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
2 * 105000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AFG3 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HiLoad Q-Sepharose column chromatography, and Superdex S200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Escherichia coli | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2-oxoglutarate decarboxylase | - |
Escherichia coli |
E1 component of the 2-oxoglutarate dehydrogenase multienzyme complex | - |
Escherichia coli |
E1o component | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent | Escherichia coli |