Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
General Stability | Organism |
---|---|
purified enzyme is highly sensitive to contaminating proteolytic activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida | |
0.147 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
0.149 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | or Mg2+, Mn2+, best activating metal ions | Pseudomonas putida | |
Mg2+ | or Mn2+, Co2+, best activating metal ions | Pseudomonas putida | |
Mn2+ | or Mg2+, Co2+, best activating metal ions | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
98082 | - |
2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
185000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | O33468 | pyruvate dehydrogenase E1 component | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87 | - |
pH 6.7, 30°C | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | propanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-oxoisovalerate + oxidized 2,6-dichlorophenolindophenol | 16% of the activity with 2-oxobutanoate | Pseudomonas putida | 2-methylpropanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
2-oxovalerate + oxidized 2,6-dichlorophenolindophenol | 49% of the activity with 2-oxobutanoate | Pseudomonas putida | butanal + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
pyruvate + oxidized 2,6-dichlorophenolindophenol | 17% of the activity with 2-oxobutanoate | Pseudomonas putida | acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 93000, SDS-PAGE, 2 * 98082, calculated | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
MdeB | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
stable up to | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
0.128 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida | |
0.17 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | Km value 0.017 mM, in presence of Mg2+ and 2-oxobutanoate | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
pyruvate | pH 6.7, 30°C | Pseudomonas putida | |
0.86 | - |
2-oxovalerate | pH 6.7, 30°C | Pseudomonas putida | |
10.7 | - |
2-oxobutanoate | pH 6.7, 30°C | Pseudomonas putida |