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Literature summary for 1.2.4.1 extracted from

  • Inoue, H.; Nishito, A.; Eriguchi, S.; Tamura, T.; Inagaki, K.; Tanaka, H.
    Purification and substrate characterization of alpha-ketobutyrate decarboxylase from Pseudomonas putida (2003), J. Mol. Catal. B, 23, 265-271.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

General Stability

General Stability Organism
purified enzyme is highly sensitive to contaminating proteolytic activity Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida
0.147
-
pyruvate pH 6.7, 30°C Pseudomonas putida
0.149
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ or Mg2+, Mn2+, best activating metal ions Pseudomonas putida
Mg2+ or Mn2+, Co2+, best activating metal ions Pseudomonas putida
Mn2+ or Mg2+, Co2+, best activating metal ions Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida
98082
-
2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida
185000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida O33468 pyruvate dehydrogenase E1 component
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87
-
pH 6.7, 30°C Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, stable for several months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + oxidized 2,6-dichlorophenolindophenol
-
Pseudomonas putida propanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
2-oxoisovalerate + oxidized 2,6-dichlorophenolindophenol 16% of the activity with 2-oxobutanoate Pseudomonas putida 2-methylpropanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
2-oxovalerate + oxidized 2,6-dichlorophenolindophenol 49% of the activity with 2-oxobutanoate Pseudomonas putida butanal + CO2 + reduced 2,6-dichlorophenolindophenol
-
?
pyruvate + oxidized 2,6-dichlorophenolindophenol 17% of the activity with 2-oxobutanoate Pseudomonas putida acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
dimer 2 * 93000, SDS-PAGE, 2 * 98082, calculated Pseudomonas putida

Synonyms

Synonyms Comment Organism
MdeB
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
stable up to Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
pyruvate pH 6.7, 30°C Pseudomonas putida
0.128
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida
0.17
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate Km value 0.017 mM, in presence of Mg2+ and 2-oxobutanoate Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.36
-
pyruvate pH 6.7, 30°C Pseudomonas putida
0.86
-
2-oxovalerate pH 6.7, 30°C Pseudomonas putida
10.7
-
2-oxobutanoate pH 6.7, 30°C Pseudomonas putida