Crystallization (Comment) | Organism |
---|---|
modeling of the beta-subunit of the pyruvate decarboxylase component of the pyruvate dehydrogenase complex based on the crystal structures of the homologous 2-oxoisovalerate decarboxylase of Pseudomonas putida and Homo sapiens. The negatively charged side chain of Glu285 and the hydrophobic side chain of Phe324 are of particular importance in the interaction with the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component of the complex | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D203A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
E251A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
E285A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain. Mutant subunit dissociates from the wild-type peripheral subunit-binding domain about 9fold faster than wild-type | Geobacillus stearothermophilus |
F324A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain. Mutant subunit dissociates from the wild-type peripheral subunit-binding domain about 13fold faster than wild-type | Geobacillus stearothermophilus |
K252A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
M322A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
N323A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
R255A | enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P21874 | beta subunit | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
wild-type, 30°C, pH not specified in the publication | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2,6-dichlorophenolindophenol | - |
Geobacillus stearothermophilus | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PdhB | - |
Geobacillus stearothermophilus |
pyruvate dehydrogenase E1 component subunit beta | - |
Geobacillus stearothermophilus |