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Literature summary for 1.2.4.1 extracted from

  • Moreau, R.; Heath, S.H.D.; Doneanu, C.E.; Harris, R.A.; Hagen, T.M.
    Age-related compensatory activation of pyruvate dehydrogenase complex in rat heart (2004), Biochem. Biophys. Res. Commun., 325, 48-58.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Rattus norvegicus
Mg2+
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40315
-
x * 40315, pyruvate dehydrogenase multienzyme complex component E1alpha, sequence calculation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus determination of free short-chain CoA esters in rat heart ?
-
?
pyruvate + CoA + NAD+ Rattus norvegicus enzyme is the E1alpha component of the pyruvate dehydrogenase multienzyme complex, regulation via reversible phosphorylation, age-related in crease in enzyme E1alpha activity due to age-related decline in pyruvate dehydrogenase kinase PDK-4, overview acetyl-CoA + CO2 + NADH
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme, as pyruvate dehydrogenase multienzyme complex component E1alpha, becomes reversibly phosphorylated at 3 serine residues by specific pyruvate dehydrogenase kinases PDK1-4, and dephosphorylated by a specific pyruvate dehydrogenase phosphatase PDP-1 and to a lesser extant PDP-2, regulatory function, pyruvate oxidation is inhibited in phosphorylated state of E1alpha Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
heart from 4-, and 24- to 28-months-old male F344 rats Rattus norvegicus
-
skeletal muscle
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information determination of free short-chain CoA esters in rat heart Rattus norvegicus ?
-
?
pyruvate + CoA + NAD+
-
Rattus norvegicus acetyl-CoA + CO2 + NADH
-
?
pyruvate + CoA + NAD+ enzyme is the E1alpha component of the pyruvate dehydrogenase multienzyme complex, regulation via reversible phosphorylation, age-related in crease in enzyme E1alpha activity due to age-related decline in pyruvate dehydrogenase kinase PDK-4, overview Rattus norvegicus acetyl-CoA + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
? x * 40315, pyruvate dehydrogenase multienzyme complex component E1alpha, sequence calculation Rattus norvegicus

Synonyms

Synonyms Comment Organism
E1alpha component of the pyruvate dehydrogenase multienzyme complex Rattus norvegicus
PDC
-
Rattus norvegicus
pyruvate dehydrogenase complex
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
thiamine diphosphate
-
Rattus norvegicus