KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40315 | - |
x * 40315, pyruvate dehydrogenase multienzyme complex component E1alpha, sequence calculation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | determination of free short-chain CoA esters in rat heart | ? | - |
? | |
pyruvate + CoA + NAD+ | Rattus norvegicus | enzyme is the E1alpha component of the pyruvate dehydrogenase multienzyme complex, regulation via reversible phosphorylation, age-related in crease in enzyme E1alpha activity due to age-related decline in pyruvate dehydrogenase kinase PDK-4, overview | acetyl-CoA + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme, as pyruvate dehydrogenase multienzyme complex component E1alpha, becomes reversibly phosphorylated at 3 serine residues by specific pyruvate dehydrogenase kinases PDK1-4, and dephosphorylated by a specific pyruvate dehydrogenase phosphatase PDP-1 and to a lesser extant PDP-2, regulatory function, pyruvate oxidation is inhibited in phosphorylated state of E1alpha | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | from 4-, and 24- to 28-months-old male F344 rats | Rattus norvegicus | - |
skeletal muscle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | determination of free short-chain CoA esters in rat heart | Rattus norvegicus | ? | - |
? | |
pyruvate + CoA + NAD+ | - |
Rattus norvegicus | acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + NAD+ | enzyme is the E1alpha component of the pyruvate dehydrogenase multienzyme complex, regulation via reversible phosphorylation, age-related in crease in enzyme E1alpha activity due to age-related decline in pyruvate dehydrogenase kinase PDK-4, overview | Rattus norvegicus | acetyl-CoA + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40315, pyruvate dehydrogenase multienzyme complex component E1alpha, sequence calculation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
E1alpha | component of the pyruvate dehydrogenase multienzyme complex | Rattus norvegicus |
PDC | - |
Rattus norvegicus |
pyruvate dehydrogenase complex | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
thiamine diphosphate | - |
Rattus norvegicus |