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Literature summary for 1.2.3.4 extracted from
- New extracellular thermostable oxalate oxidase produced from endophytic Ochrobactrum intermedium CL6: Purification and biochemical characterization (2016), Prep. Biochem. Biotechnol., 46, 734-739.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzymatic activity is not influenced by D-glucose, urea, and lactate | Brucella intermedia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not show substrate inhibition up to a oxalate concentration of 50 mM | Brucella intermedia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
oxalate | at pH 3.8 and 80°C | Brucella intermedia |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity | Brucella intermedia | |
| Fe2+ | stimulates activity | Brucella intermedia | |
| additional information | the enzymatic activity is not influenced by K+, Na+, Zn2+, Fe3+, Mn2+, and Mg2+ | Brucella intermedia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalate + O2 + H+ | Brucella intermedia | - |
CO2 + H2O2 | - |
? | |
| oxalate + O2 + H+ | Brucella intermedia CL6 | - |
CO2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brucella intermedia | - |
- |
- |
| Brucella intermedia CL6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brucella intermedia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalate + O2 + H+ | - |
Brucella intermedia | CO2 + H2O2 | - |
? | |
| oxalate + O2 + H+ | - |
Brucella intermedia CL6 | CO2 + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Brucella intermedia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 80 | the enzyme is highly stable at 4-80°C for 6 h | Brucella intermedia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 85 | - |
oxalate | at pH 3.8 and 80°C | Brucella intermedia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Brucella intermedia |
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Release 2023.1 (January 2023)
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