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Literature summary for 1.2.3.4 extracted from
- Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass (1999), Indian J. Biochem. Biophys., 36, 449-452.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose | physiological concentration, 20% activation free protein, 25% activation immobilized protein | Amaranthus spinosus |  |
| glycine | 1 mM concentration, 10% activation free protein, 18% activation immobilized protein | Amaranthus spinosus | |
| hippuric acid | 1 mM concentration, 10% activation free protein, 8% activation immobilized protein | Amaranthus spinosus | |
| serine | 1 mM concentration, 12% activation free protein, 10% activation immobilized protein | Amaranthus spinosus | |
| Urea | physiological concentration, 8% activation free protein | Amaranthus spinosus | |
| uric acid | 1 mM concentration, 12% activation free protein, 8% activation immobilized protein | Amaranthus spinosus | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | immobilized oxalate oxidase to determine oxalate concentration in urine for diagnosis of various forms of hyperoxaluria | Amaranthus spinosus |
General Stability
| General Stability | Organism |
|---|---|
| immobilized on alkylamine glass | Amaranthus spinosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | wild type and immobilized 88% and 81% inhibition respectively | Amaranthus spinosus | |
| Dithionite | - |
Amaranthus spinosus | |
| glutathione | slight inhibition, wild type and immobilized | Amaranthus spinosus | |
| L-cysteine | wild type and immobilized slight inhibition | Amaranthus spinosus | |
| NaCl | 10% inhibition at 1 mM concentration | Amaranthus spinosus | |
| nitrate | mM concentration | Amaranthus spinosus | |
| nitrite | 1 mM concentration 10% inhibition | Amaranthus spinosus | |
| pyruvate | 1 mM concentration 15% inhibition of the free enzyme, not of the immobilized enzyme | Amaranthus spinosus | |
| salicylate | - |
Amaranthus spinosus | |
| ZnSO4 | 1 mM concentration, 10% inhibition of the free enzyme, not of the immobilized enzyme | Amaranthus spinosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
oxalate | - |
Amaranthus spinosus | |
| 0.39 | - |
oxalate | immobilized protein | Amaranthus spinosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Amaranthus spinosus | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amaranthus spinosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amaranthus spinosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Amaranthus spinosus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 3 months, distilled water, free protein, complete loss of activity, immobilized protein, 70% of activity retained | Amaranthus spinosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxalic acid + O2 + 2 H+ | - |
Amaranthus spinosus | 2 CO2 + H2O2 | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
immobilized protein | Amaranthus spinosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
after 30 min wild type 60% activity, immobilized protein 27% activity | Amaranthus spinosus |
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