Literature summary for 1.2.3.3 extracted from

Li, M.; Yao, S.; Shimizu, K.
Effect of poxB gene knockout on metabolism in Escherichia coli based on growth characteristics and enzyme activities (2007), World J. Microbiol. Biotechnol., 23, 573-580.

Search for more literature for 1.2.3.3

Application

Application	Comment	Organism
additional information	pyruvate oxidase is not only a stationary-phase enzyme. Removal of the poxB gene affects the central metabolism at the enzyme level	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	poxB mutant, absence of pyruvate oxidase activity, which reduces glucose uptake rate, biomass yield, acetate production and cell growth rate, and increases O2 consumption and CO2 evolution. Glucokinase activity increases, whereas the flux through glycolysis is reduced due to down-regulation of other glycolytic enzymes such as 6-phosphofructosekinase and fructose bisphosphate aldolase. TCA cycle enzymes such as citrate synthase and malate dehydrogenase are repressed in the poxB mutant when cells are cultivated in LB medium. Activation of glucose-6-phosphate dehydrogenase and acetyl-CoA synthetase	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	strains BW25113 and JW0855	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate + H2O	-	Escherichia coli	acetate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
poxB	-	Escherichia coli

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Release 2023.1 (January 2023)