Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli strain ZK126 carrying the plasmid pYYC102 which encodes the poxB gene | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the full-length form and a proteolytically activated C-terminal truncation variant which lacks the last 23 amino acids, by the hanging-drop vapour-diffusion method using either protamine sulfate (fulllength POX) or 2-methyl-2,4-pentanediol (DELTA23 POX) as precipitants. To 2.9 A resolution. Fulllength POX crystallizes in the tetragonal space group P43212 with two monomers per asymmetric unit, the crystals of DELTA23 POX belong to the orthorhombic space group P212121 and contain 12 monomers per asymmetric unit | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography, more than 95% pure | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + phosphate + ferricyanide + H2O | - |
Escherichia coli | acetyl phosphate + ferrocyanide + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
POX | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |