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Literature summary for 1.2.3.3 extracted from

  • Wille, G.; Ritter, M.; Friedemann, R.; Mantele, W.; Hubner, G.
    Redox-triggered FTIR difference spectra of FAD in aqueous solution and bound to flavoproteins (2003), Biochemistry, 42, 14814-14821.
    View publication on PubMed
Search for more literature for 1.2.3.3

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant V265A Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
V265A binds FAD more weakly Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Lactiplantibacillus plantarum
AMP
-
 Lactiplantibacillus plantarum
ATP
-
 Lactiplantibacillus plantarum
NAD+
-
 Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + phosphate + O2
-
 Lactiplantibacillus plantarum acetyl phosphate + CO2 + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.5
-
 pyruvate pH 6.0, 25°C, mutant V265A Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
FAD bound to V265 Lactiplantibacillus plantarum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
 ATP pH 6.0, 25°C, mutant V265A Lactiplantibacillus plantarum
1.5
-
 ADP pH 6.0, 25°C, mutant V265A Lactiplantibacillus plantarum
3
-
 AMP pH 6.0, 25°C, mutant V265A Lactiplantibacillus plantarum
6
-
 NAD+ pH 6.0, 25°C, mutant V265A Lactiplantibacillus plantarum