Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 11% inhibition at 10 mM | Lactiplantibacillus plantarum | |
KCN | 25% inhibition at 10 mM | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
pyruvate | electron acceptor: oxygen | Lactiplantibacillus plantarum | |
1.25 | - |
arsenate | electron acceptor: oxygen | Lactiplantibacillus plantarum | |
2.3 | - |
phosphate | electron acceptor: oxygen | Lactiplantibacillus plantarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | most effective | Lactiplantibacillus plantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE | Lactiplantibacillus plantarum |
262000 | - |
gel filtration | Lactiplantibacillus plantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + phosphate + O2 + H2O | Lactiplantibacillus plantarum | - |
acetyl phosphate + CO2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, ultracentrifugation and chromatography techniques | Lactiplantibacillus plantarum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Lactiplantibacillus plantarum | - |
Storage Stability | Organism |
---|---|
4°C, pH 5.7, several weeks | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + phosphate + O2 + H2O | 15% of the activity compared to pyruvate as substrate | Lactiplantibacillus plantarum | ? + H2O2 | - |
? | |
alpha-ketobutyrate + phosphate + O2 + H2O | very low activity | Lactiplantibacillus plantarum | ? + H2O2 | - |
? | |
methylglyoxal + phosphate + O2 + H2O | 20% of the activity compared to pyruvate as substrate | Lactiplantibacillus plantarum | ? + H2O2 | - |
? | |
pyruvate + arsenate + O2 + H2O | can replace phosphate, 25% higher activity compared to phosphate as cosubstrate | Lactiplantibacillus plantarum | acetyl arsenate + CO2 + H2O2 | - |
? | |
pyruvate + phosphate + 2,6-dichlorophenolindophenol + H2O | 36% of the activity compared to oxygen as electron acceptor | Lactiplantibacillus plantarum | acetyl phosphate + CO2 + reduced 2,6-dichlorophenolindophenol + H2O2 | - |
? | |
pyruvate + phosphate + ferricyanide + H2O | 65% of the activity compared to oxygen as electron acceptor | Lactiplantibacillus plantarum | acetyl phosphate + ferrocyanide + H2O2 | - |
? | |
pyruvate + phosphate + O2 + H2O | - |
Lactiplantibacillus plantarum | acetyl phosphate + CO2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, SDS-PAGE | Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Lactiplantibacillus plantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
unstable above | Lactiplantibacillus plantarum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 5.8 | highest stability at 4°C and highest remaining activity of 67% | Lactiplantibacillus plantarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | tightly bound | Lactiplantibacillus plantarum | |
thiamine diphosphate | tightly bound | Lactiplantibacillus plantarum |