Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Arthrobacter globiformis | |
cysteine | - |
Arthrobacter globiformis | |
dithiothreitol | - |
Arthrobacter globiformis | |
GSH | optimal activity at 0.002 mM, can be replaced by 2-mercaptoethanol, cysteine or dithiothreitol | Arthrobacter globiformis |
General Stability | Organism |
---|---|
stabilizing effects of activity with ammonium sulfate, NaCl, Na2SO4 or MgSO4 | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | potent inhibitor, other metal chelators ineffective | Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal activity at 0.01 mM | Arthrobacter globiformis | |
Mn2+ | optimal activity at 0.01 mM | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Arthrobacter globiformis | - |
4-hydroxyphenylacetate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | Arthrobacter globiformis PRL W15 | - |
4-hydroxyphenylacetate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:L-tyrosine-grown cell | - |
Arthrobacter globiformis | - |
Storage Stability | Organism |
---|---|
-20°C, ammonium sulfate precipitation or crude extract, 4 weeks | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Arthrobacter globiformis | 4-hydroxyphenylacetate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | - |
Arthrobacter globiformis PRL W15 | 4-hydroxyphenylacetate + CO2 | - |
? | |
glyoxylate + O2 | 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis | CO2 + CO2 | - |
? | |
glyoxylate + O2 | 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis PRL W15 | CO2 + CO2 | - |
? | |
phenylpyruvate + O2 | 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis | phenylacetate + CO2 | - |
? | |
phenylpyruvate + O2 | 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis PRL W15 | phenylacetate + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
10% loss of activity per h | Arthrobacter globiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | optimal activity at 10 nM, can not be replaced by NAD+, NADP+ or FMN | Arthrobacter globiformis | |
thiamine diphosphate | optimal activity at 0.004 mM, absolute requirement | Arthrobacter globiformis |