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Literature summary for 1.2.3.13 extracted from

  • Blakley, E.R.
    The catabolism of L-tyrosine by an Arthrobacter sp. (1977), Can. J. Microbiol., 23, 1128-1139.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Arthrobacter globiformis
cysteine
-
Arthrobacter globiformis
dithiothreitol
-
Arthrobacter globiformis
GSH optimal activity at 0.002 mM, can be replaced by 2-mercaptoethanol, cysteine or dithiothreitol Arthrobacter globiformis

General Stability

General Stability Organism
stabilizing effects of activity with ammonium sulfate, NaCl, Na2SO4 or MgSO4 Arthrobacter globiformis

Inhibitors

Inhibitors Comment Organism Structure
EDTA potent inhibitor, other metal chelators ineffective Arthrobacter globiformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activity at 0.01 mM Arthrobacter globiformis
Mn2+ optimal activity at 0.01 mM Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylpyruvate + O2 Arthrobacter globiformis
-
4-hydroxyphenylacetate + CO2
-
?
4-hydroxyphenylpyruvate + O2 Arthrobacter globiformis PRL W15
-
4-hydroxyphenylacetate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:L-tyrosine-grown cell
-
Arthrobacter globiformis
-

Storage Stability

Storage Stability Organism
-20°C, ammonium sulfate precipitation or crude extract, 4 weeks Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
Arthrobacter globiformis 4-hydroxyphenylacetate + CO2
-
?
4-hydroxyphenylpyruvate + O2
-
Arthrobacter globiformis PRL W15 4-hydroxyphenylacetate + CO2
-
?
glyoxylate + O2 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate Arthrobacter globiformis CO2 + CO2
-
?
glyoxylate + O2 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate Arthrobacter globiformis PRL W15 CO2 + CO2
-
?
phenylpyruvate + O2 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate Arthrobacter globiformis phenylacetate + CO2
-
?
phenylpyruvate + O2 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate Arthrobacter globiformis PRL W15 phenylacetate + CO2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
10% loss of activity per h Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FAD optimal activity at 10 nM, can not be replaced by NAD+, NADP+ or FMN Arthrobacter globiformis
thiamine diphosphate optimal activity at 0.004 mM, absolute requirement Arthrobacter globiformis