Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pyridine | non-linear inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
phenanthridine | methanol, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.00625 | - |
phenanthridine | pyridine, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.0063 | - |
phenanthridine | acetonitrile, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.00666 | - |
phenanthridine | dioxane, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.00769 | - |
phenanthridine | 2-propanol, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.00833 | - |
phenanthridine | 1-propanol, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.00833 | - |
phenanthridine | ethanol, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.01 | - |
phenanthridine | N-N-dimethylformamide, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.011 | - |
phenanthridine | phosphate buffer, pH 7.4, at 35°C | Oryctolagus cuniculus | |
0.011 | - |
phenanthridine | tetrahydrofuran, pH 7.4, at 35°C | Oryctolagus cuniculus |
Organic Solvent | Comment | Organism |
---|---|---|
Methanol | concentration of half inactivation = 0.5% | Oryctolagus cuniculus |
additional information | in the case of all organic solvents, the activity of aldehyde oxidase decreased when the concentration of the solvent increased in the reaction medium | Oryctolagus cuniculus |
N,N-dimethylformamide | concentration of half inactivation = 13.3% | Oryctolagus cuniculus |
Pyridine | concentration of half inactivation = 0.5% | Oryctolagus cuniculus |
tetrahydrofuran | concentration of half inactivation = 0.5% | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P80456 | New Zealand rabbit | - |
Purification (Comment) | Organism |
---|---|
partially purified by heat treatment and ammonium sulfate precipitation | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in the case of all organic solvents, the activity of aldehyde oxidase decreases when the concentration of the solvent increases in the reaction medium | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenanthridine + H2O + O2 | activity of rabbit liver aldehyde oxidase characterized in nine water-miscible organic mixtures i.e. phosphate buffer mixed with N-N-dimethylformamide, acetonitrile, tetrahydrofuran, 1-propanol, 2-propanol, ethanol, pyridine, dioxane, and methanol | Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AOX1 | - |
Oryctolagus cuniculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
reduction in the enzyme activity at all temperatures in the presence of all organic solvents, except methanol and acetonitrile, enzyme activity in the presence of these two solvents increases with a rise in temperature up to 45°C followed by a marked reduction at 55°C, behavior of aldehyde oxidase is similar to that in the organic solvent free buffer solution | Oryctolagus cuniculus |
25 | 55 | in the organic solvent-free buffer, a significant increase in the aldehyde oxidase activity observable as temperature raises up to 45°C while enzyme activity decreases almost to zero when the reaction temperature is elevated to 55°C | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
incubation of aldehyde oxidase in all water-miscible organic solvents results in a marked loss of the catalytic activity, residual activity of aldehyde oxidase in the presence of dioxane, ethanol, N,N-dimethylformamide, and pyridine is 16%, 10%, 12%, and 17% after 24 h at 35°C | Oryctolagus cuniculus |
35 | - |
the activity remains almost unchanged after 24 h in the absence of organic solvents at 35°C, enzyme is relatively stable in the presence of methanol, 2-propanol and acetonitrile | Oryctolagus cuniculus |
55 | - |
at 55°C, the enzyme undergoes a conformational change producing an inactive form, conversion is reversible | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the manner of the enzyme activity in the hydro-organic mixtures at different pHs is almost similar to that observed in the phosphate buffer system | Oryctolagus cuniculus |
9 | - |
phosphate buffer with no added organic solvent shows the highest aldehyde oxidase activity | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | aldehyde oxidase active in either the organic solvent-free buffer or all nine water-miscible organic solvents, lowest enzyme activity is obtained at pH 6.0 with acetonitrile | Oryctolagus cuniculus |