Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E1265Q | catalytically inactive, residue E1265 initiates the base-catalyzed mechanism of substrate oxidation | Mus musculus |
M884R | drastic decrease in the oxidation of aldehydes, with no increase in the oxidation of purine substrates | Mus musculus |
V806E | drastic decrease in the oxidation of aldehydes, with no increase in the oxidation of purine substrates | Mus musculus |
V806E/M884R | catalytically inactive | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
retinaldehyde | mutant M884R, 30°C, pH 7.4 | Mus musculus | |
0.0114 | - |
Phthalazine | wild-type, 30°C, pH 7.4 | Mus musculus | |
0.0222 | - |
retinaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
0.0285 | - |
Phthalazine | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
0.0558 | - |
retinaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus | |
0.0977 | - |
benzaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus | |
0.5199 | - |
acetaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus | |
0.6345 | - |
benzaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
7.13 | - |
benzaldehyde | mutant M884R, 30°C, pH 7.4 | Mus musculus | |
52.9 | - |
acetaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O54754 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aldehyde + H2O + O2 = a carboxylate + H2O2 | base catalyzed mechanism. Residue E1265 acts as an active site base that abstracts a proton from the Mo-OH group, which in turn undertakes a nucleophilic attack on the substrate benzaldehyde. After hydride transfer to the Mo= S group, the initial intermediate breaks down, with the transient formation of a paramagnetic MoV species, followed by displacement of product by a water molecule to return to the starting LMoVIOS(OH) state. The roles of residues Met884 and Val806 are stabilization of substrate binding | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + H2O + O2 | - |
Mus musculus | acetic acid + H2O2 | - |
? | |
benzaldehyde + H2O + O2 | - |
Mus musculus | benzoate + H2O2 | - |
? | |
additional information | no substrate: xanthine, hypoxanthine | Mus musculus | ? | - |
? | |
phthalazine + H2O + O2 | - |
Mus musculus | ? + H2O2 | - |
? | |
retinaldehyde + O2 + H2O | - |
Mus musculus | retinoic acid + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
retinaldehyde | mutant M884R, 30°C, pH 7.4 | Mus musculus | |
0.19 | - |
Phthalazine | wild-type, 30°C, pH 7.4 | Mus musculus | |
0.23 | - |
retinaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
0.83 | - |
retinaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus | |
1.27 | - |
benzaldehyde | mutant M884R, 30°C, pH 7.4 | Mus musculus | |
1.72 | - |
Phthalazine | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
2.52 | - |
benzaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
2.7 | - |
acetaldehyde | mutant V806E, 30°C, pH 7.4 | Mus musculus | |
5.29 | - |
benzaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus | |
8.66 | - |
acetaldehyde | wild-type, 30°C, pH 7.4 | Mus musculus |