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Literature summary for 1.2.3.1 extracted from

  • Sasaki, Y.; Isobe, K.; Kataoka, M.; Ogawa, J.; Iwasaki, A.; Hasegawa, J.; Shimizu, S.
    Purification and characterization of a new aldehyde oxidase from Pseudomonas sp. AIU 362 (2008), J. Biosci. Bioeng., 106, 297-302.
    View publication on PubMed
Search for more literature for 1.2.3.1

Activating Compound

Activating Compound Comment Organism Structure
Sodium azide 1 mM, 128% of initial activity Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
hydrazine 1 mM, 28% residual activity Pseudomonas sp.
hydroxyamine 1 mM, 52% residual activity Pseudomonas sp.
MgCl2 1 mM, 62% residual activity Pseudomonas sp.
phenylhydrazine 1 mM, 12% residual activity Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00014
-
 hexylaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0005
-
 Butyraldehyde 30°C, pH 7.0 Pseudomonas sp.
0.00057
-
 benzaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0008
-
 p-Methoxybenzaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0011
-
 Valeraldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0025
-
 acetaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0026
-
 p-Hydroxybenzaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0028
-
 propionaldehyde 30°C, pH 7.0 Pseudomonas sp.
0.0097
-
 Glyoxal 30°C, pH 7.0 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl2 1 mM, 123% of initial activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 4 * 27000, SDS-PAGE Pseudomonas sp.
95000
-
 PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. AIU 362
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + H2O + O2
-
 Pseudomonas sp. acetic acid + H2O2
-
 ?
acetaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 acetic acid + H2O2
-
 ?
benzaldehyde + H2O + O2
-
 Pseudomonas sp. benzoate + H2O2
-
 ?
benzaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 benzoate + H2O2
-
 ?
butyraldehyde + H2O + O2
-
 Pseudomonas sp. butanoate + H2O2
-
 ?
glyoxal + H2O + O2
-
 Pseudomonas sp. glyoxylate + H2O2
-
 ?
hexylaldehyde + H2O + O2
-
 Pseudomonas sp. hexanoate + H2O2
-
 ?
hexylaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 hexanoate + H2O2
-
 ?
p-hydroxybenzaldehyde + H2O + O2
-
 Pseudomonas sp. p-hydroxybenzoate + H2O2
-
 ?
p-methoxybenzaldehyde + H2O + O2
-
 Pseudomonas sp. p-methoxybenzoate + H2O2
-
 ?
propionaldehyde + H2O + O2
-
 Pseudomonas sp. propionic acid + H2O2
-
 ?
propionaldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 propionic acid + H2O2
-
 ?
valeraldehyde + H2O + O2
-
 Pseudomonas sp. valeric acid + H2O2
-
 ?
valeraldehyde + H2O + O2
-
 Pseudomonas sp. AIU 362 valeric acid + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 55 enzyme retains at least 80% of initial activity for 30 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate glyoxal Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5 30°C, retains more than 80% of initial activity for at least 2 h Pseudomonas sp.