Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric cDNAs by mutual exchange of 2Fe-2S/FAD and MoCo domains between cynomolgous monkey and rat. Chimeric monkey/rat AO is referred to one with monkey type 2Fe-2S/FAD domains and a rat type MoCo domain. Rat/monkey AO is vice versa. Substrate inhibition is seen in rat AO and chimeric monkey/rat AO, but not in monkey AO and chimeric rat/monkey AO. A biphasic Eadie-Hofstee profile is observed in monkey AO and chimeric rat/monkey AO, but not rat AO and chimeric monkey/rat AO. Two-fold greater Vmax values are observed in monkey AO than in chimeric rat/monkey AO, and in chimeric monkey/rat AO than in rat AO | Rattus norvegicus |
additional information | construction of chimeric cDNAs by mutual exchange of 2Fe-2S/FAD and MoCo domains between cynomolgous monkey and rat. Chimeric monkey/rat AO is referred to one with monkey type 2Fe-2S/FAD domains and a rat type MoCo domain. Rat/monkey AO is vice versa. Substrate inhibition is seen in rat AO and chimeric monkey/rat AO, but not in monkey AO and chimeric rat/monkey AO. A biphasic EadieHofstee profile is observed in monkey AO and chimeric rat/monkey AO, but not rat AO and chimeric monkey/rat AO. Two-fold greater Vmax values are observed in monkey AO than in chimeric rat/monkey AO, and in chimeric monkey/rat AO than in rat AO | Macaca fascicularis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric rat/monkey enzyme, high affinity value, pH 6.0, 37°C | Rattus norvegicus | |
0.0043 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric rat/monkey enzyme, high affinity value, pH 6.0, 37°C | Macaca fascicularis | |
0.0164 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | wild-type, low affinity value, pH 6.0, 37°C | Macaca fascicularis | |
0.0171 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric rat/monkey enzyme, low affinity value, pH 6.0, 37°C | Rattus norvegicus | |
0.0171 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric rat/monkey enzyme, low affinity value, pH 6.0, 37°C | Macaca fascicularis | |
0.0351 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | wild-type, high affinity value, pH 6.0, 37°C | Macaca fascicularis | |
0.0363 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric monkey/rat enzyme, pH 6.0, 37°C | Rattus norvegicus | |
0.0363 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | chimeric monkey/rat enzyme, pH 6.0, 37°C | Macaca fascicularis | |
0.0528 | - |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine | wild-type, pH 6.0, 37°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca fascicularis | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 | i.e. RS-8359, 2-oxidation of the S-enantiomer | Rattus norvegicus | ? + H2O2 | - |
? | |
4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta[d]-pyrimidine + H2O + O2 | i.e. RS-8359, 2-oxidation of the S-enantiomer | Macaca fascicularis | ? + H2O2 | - |
? |