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Literature summary for 1.2.3.1 extracted from

  • Hoshino, K.; Itoh, K.; Masubuchi, A.; Adachi, M.; Asakawa, T.; Watanabe, N.; Kosaka, T.; Tanaka, Y.
    Cloning, expression, and characterization of male cynomolgus monkey liver aldehyde oxidase (2007), Biol. Pharm. Bull., 30, 1191-1198.
    View publication on PubMed
Search for more literature for 1.2.3.1

Application

Application Comment Organism
additional information 96% amino acid identity with those of human enzyme. Two forms of aldehyde oxidase in monkey are the expression products by a single gene due to possibly existence of two aldehyde oxidase isoforms or two active sites in a single enzyme Macaca fascicularis

Cloned(Commentary)

Cloned (Comment) Organism
into the pQE30Xa vector and expressed in Escherichia coli M15 (pREP4) cells Macaca fascicularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 (S)-RS-8359 high affinity component of the expressed enzyme Macaca fascicularis
0.0238
-
 (S)-RS-8359
-
 Rattus norvegicus
0.0754
-
 (S)-RS-8359 low affinity component of the expressed enzyme Macaca fascicularis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Macaca fascicularis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 130000, SDS-PAGE, x * 150000, Coomassie brilliant blue-staining Macaca fascicularis
150000
-
 x * 130000, SDS-PAGE, x * 150000, Coomassie brilliant blue-staining Macaca fascicularis
250000
-
 gel filtration Macaca fascicularis
300000
-
 Western blot analysis Macaca fascicularis

Organism

Organism UniProt Comment Textmining
Macaca fascicularis Q5FB27
-
-
Rattus norvegicus
-
 Donryu strain rats
-

Purification (Commentary)

Purification (Comment) Organism
231fold purified at a yield of about 1% by heat treatment, ammonium sulfate fractionation, column chromatographies, and hydroxyapatite Macaca fascicularis

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Macaca fascicularis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8153
-
 cytosolic enzyme Macaca fascicularis
188.3
-
 231fold purified enzyme Macaca fascicularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-RS-8359 enzyme shows a biphasic pattern in Eadie–Hofstee plots of the (S)-enantiospecific 2-oxidation activity of RS-8359 Macaca fascicularis ?
-
 ?
(S)-RS-8359 enzyme shows a monophasic pattern in Eadie-Hofstee plots of the (S)-enantiospecific 2-oxidation activity of RS-8359 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, SDS-PAGE, x * 150000, Coomassie brilliant blue-staining Macaca fascicularis