Any feedback?
Literature summary for 1.2.3.1 extracted from
- Purification and properties of flavin- and molybdenum-containing aldehyde oxidase from coleoptiles of maize (1996), Plant Physiol., 110, 781-789.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 40% inhibition at 5 mM | Zea mays |  |
| iodoacetate | 31% inhibition at 1 mM | Zea mays | |
| p-chloromercuribenzoic acid | 61% inhibition at 0.05 mM | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
benzaldehyde | O2 as electron acceptor | Zea mays | |
| 0.0029 | - |
Protocatechualdehyde | O2 as electron acceptor | Zea mays | |
| 0.0032 | - |
Indole-3-acetaldehyde | O2 as electron acceptor | Zea mays | |
| 0.0045 | - |
Indole-3-aldehyde | O2 as electron acceptor | Zea mays | |
| 0.005 | - |
benzaldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.005 | - |
Indole-3-acetaldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.014 | - |
Indole-3-aldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.026 | - |
Butyraldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.026 | - |
Protocatechualdehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.074 | - |
propionaldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.25 | - |
phenylacetaldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
| 0.345 | - |
acetaldehyde | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Zea mays | |
| Mo5+ | - |
Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
alpha2, 2 * 150000, SDS-PAGE | Zea mays |
| 300000 | - |
gel filtration | Zea mays |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Methanol | 27% activity with 2% v/v methanol | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| coleoptile | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.137 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 20 mM Tris-HCl buffer, pH 8.0 | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + H2O + O2 | - |
Zea mays | acetate + H2O2 | - |
r | |
| benzaldehyde + H2O + O2 | 2,6-dichlorophenol-indophenol also as electron acceptor | Zea mays | benzoic acid + H2O2 | - |
? | |
| butyraldehyde + H2O + O2 | - |
Zea mays | butanoate + H2O2 | - |
r | |
| indole 3-acetaldehyde + H2O + O2 | 2,6-dichlorophenol-indophenol also as electron acceptor | Zea mays | indole 3-acetic acid + H2O2 | - |
? | |
| indole 3-carbaldehyde + H2O + O2 | 2,6-dichlorophenol-indophenol also as electron acceptor | Zea mays | indole 3-carboxylate + H2O2 | - |
? | |
| additional information | 3(4,5-dimethylthiazolyl-2)2,5-diphenyltetrazolium-bromide, ferricyanide and cytochrome c can also act as electron acceptors, the latter two are not so efficient | Zea mays | ? | - |
? | |
| phenylacetaldehyde + H2O + O2 | 2,6-dichlorophenol-indophenol as electron acceptor | Zea mays | phenylacetic acid + H2O2 | - |
? | |
| propionaldehyde + H2O + O2 | - |
Zea mays | propionate + H2O2 | - |
r | |
| protocatechualdehyde + H2O + O2 | 2,6-dichlorophenol-indophenol also as electron acceptor | Zea mays | protocatechuic acid + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 150000, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AO | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | relatively stable | Zea mays |
| 100 | - |
after 5 min complete activity loss | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Zea mays | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
